Document Detail


Tracking mechanics and volume of globular cells with atomic force microscopy using a constant-height clamp.
MedLine Citation:
PMID:  22222789     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
To understand the role of physical forces at a cellular level, it is necessary to track mechanical properties during cellular processes. Here we present a protocol that uses flat atomic force microscopy (AFM) cantilevers clamped at constant height, and light microscopy to measure the resistance force, mechanical stress and volume of globular animal cells under compression. We describe the AFM and cantilever setup, live cell culture in the AFM, how to ensure stability of AFM measurements during medium perfusion, integration of optical microscopy to measure parameters such as volume and track intracellular dynamics, and interpretation of the physical parameters measured. Although we use this protocol on trypsinized interphase and mitotic HeLa cells, it can also be applied to other cells with a relatively globular shape, especially animal cells in a low-adhesive environment. After a short setup phase, the protocol can be used to investigate approximately one cell per hour.
Authors:
Martin P Stewart; Yusuke Toyoda; Anthony A Hyman; Daniel J Müller
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Publication Detail:
Type:  Journal Article     Date:  2012-01-05
Journal Detail:
Title:  Nature protocols     Volume:  7     ISSN:  1750-2799     ISO Abbreviation:  Nat Protoc     Publication Date:  2012  
Date Detail:
Created Date:  2012-01-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101284307     Medline TA:  Nat Protoc     Country:  England    
Other Details:
Languages:  eng     Pagination:  143-54     Citation Subset:  IM    
Affiliation:
1] ETH Zurich, Department of Biosystems Science and Engineering, Basel, Switzerland. [2] Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
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