Document Detail


Tracking the glycerophospholipid distribution of docosahexaenoic acid by shotgun lipidomics.
MedLine Citation:
PMID:  19763469     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Docosahexaenoic acid (DHA, 22:6 n-3) is an omega-3 fatty acid with a 22 carbon acyl chain containing six cis double bonds and is predominantly found in membrane glycerophospholipids. Dietary consumption of DHA has been positively linked with the prevention of numerous pathologies and consequently, it has been the focus of extensive research over the last four decades. Nevertheless, our understanding of its molecular mode of action is not well understood. One likely mechanism is through DHA's influence on cell membranes and the proteins embedded within them. This influence may be altered depending on the glycerophospholipid head group DHA is esterified to and its fatty acid partner, i.e., the specific glycerophospholipid molecule. Accordingly, an understanding of the exact glycerophospholipid distribution of DHA within a tissue is important if we wish to gain further insight into its role in the prevention of disease. In this chapter a rapid, shotgun lipidomic approach for identifying the molecular glycerophospholipid distribution of DHA is described.
Authors:
Todd W Mitchell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  579     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2009  
Date Detail:
Created Date:  2009-09-18     Completed Date:  2010-01-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  19-31     Citation Subset:  IM    
Affiliation:
School of Health Sciences, University of Wollongong, Wollongong, NSW, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Docosahexaenoic Acids / chemistry,  metabolism*
Glycerophospholipids / chemistry,  metabolism*
Mice
Molecular Structure
Muscle, Skeletal / metabolism
Spectrometry, Mass, Electrospray Ionization
Spectrophotometry, Ultraviolet
Chemical
Reg. No./Substance:
0/Glycerophospholipids; 25167-62-8/Docosahexaenoic Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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