| Tracking hydrogen/deuterium exchange at glycan sites in glycoproteins by mass spectrometry. | |
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MedLine Citation:
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PMID: 21863800 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hydrogen/deuterium exchange coupled to mass spectrometry (HDX-MS) has emerged as a technique for studying glycoproteins, which are often refractory to classical methods. Glycan chains are generally assumed to exchange protons very rapidly, making them invisible to this technique. Here, we show that under conditions commonly used for HDX-MS, acetamido groups within glycan chains retain a significant amount of deuterium. Using mono- and polysaccharide standards along with glycopeptides from a panel of glycoproteins, we demonstrate that N-acetyl hexosamines, along with modified Asn side chains, are responsible for this effect. Model compounds for sialic acid also displayed similar exchange kinetics, but terminal sialic acids in the context of an entire glycan chain did not contribute to deuterium retention. Furthermore, the presence of sialic acid appears to enhance the exchange rate of the nearby N-acetyl glucosamines. The ability to detect deuterium exchange at the glycan level opens the possibility of applying HDX-MS to monitor glycan interactions and dynamics. |
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Authors:
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M Guttman; M Scian; K K Lee |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-09-06 |
Journal Detail:
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Title: Analytical chemistry Volume: 83 ISSN: 1520-6882 ISO Abbreviation: Anal. Chem. Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-09-30 Completed Date: 2012-01-30 Revised Date: 2012-02-07 |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
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Languages: eng Pagination: 7492-9 Citation Subset: IM |
Affiliation:
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Department of Medicinal Chemistry, University of Washington, Seattle, Washington 98195, USA. mguttman@uw.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbohydrate Conformation Cattle Deuterium Exchange Measurement* Glycoproteins / chemistry* Humans Mass Spectrometry Polysaccharides / chemistry* |
| Grant Support | |
ID/Acronym/Agency:
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K99 GM080352-01/GM/NIGMS NIH HHS; K99 GM080352-02/GM/NIGMS NIH HHS; P30AI027767/AI/NIAID NIH HHS; R00 GM080352-03/GM/NIGMS NIH HHS; R00 GM080352-04/GM/NIGMS NIH HHS; R00 GM080352-05/GM/NIGMS NIH HHS; R00GM080352/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Glycoproteins; 0/Polysaccharides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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