Document Detail

Tracking retinal microgliosis in models of retinal ganglion cell damage.
MedLine Citation:
PMID:  22879415     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To investigate the longitudinal profiles of microgliosis after optic nerve injury induced by optic nerve crush and acute elevation of intraocular pressure (IOP).
METHODS: A confocal scanning laser ophthalmoscope was used to image the retinal microglia of the CX3CR1(GFP/+) transgenic mice in vivo at baseline, 3 days and then weekly for 4 weeks after optic nerve crush (n = 3), and after elevating the IOP to 110 mm Hg for 30 (n = 3) or 60 (n = 3) minutes.
RESULTS: After optic nerve crush, the density of microglia increased by 2.43 ± 0.19-fold at week 1 and then gradually declined with 2.04 ± 0.24-, 1.69 ± 0.25-, and 1.29 ± 0.11-fold increases at week 2, 3, and 4, respectively. Microgliosis followed a similar pattern after acute IOP elevation and the increase in microglia was associated with the duration of IOP elevation. There were 1.35 ± 0.17- and 2.03 ± 0.08-fold increases in microglia at week 1, and 1.15 ± 0.11- and 1.11 ± 0.10-fold increases at week 4, after 30 and 60 minutes of acute IOP elevation, respectively. The morphology of microglia changed from ramified to ameboid form in 1 week, and then returned to ramified form in the subsequent weeks. There was a significant negative association between the number of surviving retinal ganglion cells (RGCs) and the extent of microgliosis during the follow-up period (R² = 0.72, P = 0.004).
CONCLUSIONS: Longitudinal in vivo imaging of the retinal microglia can provide an effective approach to study microgliosis and its association with RGC degeneration.
Shu Liu; Zhi-wai Li; Robert N Weinreb; Guihua Xu; James D Lindsey; Cong Ye; Wing-ho Yung; Chi-Pui Pang; Dennis Shun Chiu Lam; Christopher Kai-shun Leung
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-09-14
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  53     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2012  
Date Detail:
Created Date:  2012-09-17     Completed Date:  2012-11-26     Revised Date:  2013-01-21    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6254-62     Citation Subset:  IM    
Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong, People's Republic of China.
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MeSH Terms
Gliosis / etiology,  pathology*,  physiopathology
Intraocular Pressure / physiology
Longitudinal Studies
Mice, Transgenic
Microglia / pathology*
Microscopy, Confocal
Optic Nerve Injuries / complications,  pathology*
Retinal Ganglion Cells / pathology*
Comment In:
Invest Ophthalmol Vis Sci. 2012 Dec;53(13):8104   [PMID:  23239867 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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