Document Detail

Tracing the slow growth of anaerobic methane-oxidizing communities by (15)N-labelling techniques.
MedLine Citation:
PMID:  18269633     Owner:  NLM     Status:  MEDLINE    
The anaerobic oxidation of methane (AOM) is an important methane sink in marine ecosystems mediated by still uncultured Archaea. We established an experimental system to grow AOM communities in different sediment samples. Approaches to show growth of the slow-growing anaerobic methanotrophs have been either via nucleic acids (quantitative PCR) or required long-term incubations. Previous long-term experiments with (13)C-labelled methane led to an unspecific distribution of the (13)C-label. Although quantitative PCR is a sensitive technique to detect small changes in community composition, it does not determine growth yield. Therefore, we tested an alternative method to detect a biomass increase of AOM microorganisms with (15)N-labelled ammonium as N-source. After only 3 weeks, significant (15)N-labelling became apparent in amino acids as major structural units of microbial proteins. This was especially evident in methane-containing incubations, showing the methane-dependent uptake of the (15)N-labelled ammonium by microorganisms. Cell counts demonstrated a two- and fourfold increase at ambient or elevated methane concentrations. With denaturing gradient gel electrophoresis, over 6 months incubation no changes in community composition of sulphate-reducing bacteria and archaea were detected. These data indicate doubling times for AOM microorganisms between 2 and 3.4 months. In conclusion, the (15)N-labelling approach proved to be a sensitive and fast way to show growth of extremely slow-growing microorganisms.
Martin Krüger; Heike Wolters; Matthias Gehre; Samantha B Joye; Hans-Hermann Richnow
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology ecology     Volume:  63     ISSN:  0168-6496     ISO Abbreviation:  FEMS Microbiol. Ecol.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-13     Completed Date:  2008-04-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8901229     Medline TA:  FEMS Microbiol Ecol     Country:  England    
Other Details:
Languages:  eng     Pagination:  401-11     Citation Subset:  IM    
Geomicrobiology, Federal Institute for Geosciences and Natural Resources , Hannover, Germany.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AJ756440;  AJ756441;  AJ756442;  AJ756443;  AJ756444;  AJ756445;  AJ756446;  AJ756447;  AJ756448;  AJ756449;  AJ756450;  AJ756451;  AJ756452;  AJ756453;  AJ756454;  AJ756455;  AJ756456;  AJ756457;  AJ756458;  AJ756459
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MeSH Terms
Archaea / genetics,  growth & development*,  metabolism
Bacteria / genetics,  growth & development*,  metabolism
Culture Media
Geologic Sediments / microbiology*
Methane / metabolism*
Molecular Sequence Data
Nitrogen Isotopes / metabolism*
Sequence Analysis, DNA
Reg. No./Substance:
0/Culture Media; 0/Nitrogen Isotopes; 74-82-8/Methane

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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