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Tracing the Conversion Process for Primordial Germ Cells to Pluripotent Stem Cells in Mice.
MedLine Citation:
PMID:  22423052     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
To understand mechanisms underlying acquisition of pluripotency, it is critical to identify cells that can be converted to pluripotent stem cells. For this purpose, we focused on unipotent primordial germ cells (PGCs), which can be reprogrammed into pluripotent embryonic germ (EG) cells under defined conditions. Treatment of PGCs with combinations of signaling inhibitors, including inhibitors of MAP2K (MEK), GSK3B (GSK-3beta), and TGFB (TGF-beta) type-1 receptors, induced cells to enter a pluripotent state at high frequency (12.1%) by Day 10 of culture. When we employed fluorescence-activated cell sorting (FACS) to monitor conversion of candidate cells to a pluripotent state, we observed a cell cycle shift to S-phase, indicating enrichment of pluripotent cells, during the early phase of EG formation. Transcriptome analysis revealed that PGCs retained expression of some pluripotent stem cell-associated genes, such as Pou5f1 and Sox2, during EG cell formation. On the other hand, PGCs lost their germ lineage characteristics and acquired expression of pluripotent stem cell markers such as Klf4 and Eras. The overall gene expression profiles revealed by this system provide novel insight into how pluripotency is acquired in germ-committed cells.
Authors:
Go Nagamatsu; Takeo Kosaka; Shigeru Saito; Keiyo Takubo; Hideo Akiyama; Tetsuo Sudo; Katsuhisa Horimoto; Mototsugu Oya; Toshio Suda
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-14
Journal Detail:
Title:  Biology of reproduction     Volume:  -     ISSN:  1529-7268     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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