Document Detail


Tracheal Basal cells: a facultative progenitor cell pool.
MedLine Citation:
PMID:  20522644     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Analysis of lineage relationships in the naphthalene-injured tracheal epithelium demonstrated that two multipotential keratin 14-expressing cells (K14ECs) function as progenitors for Clara and ciliated cells. These K14EC were distinguished by their self-renewal capacity and were hypothesized to reside at the stem and transit amplifying tiers of a tissue-specific stem cell hierarchy. In this study, we used gene expression and histomorphometric analysis of the steady-state and naphthalene-injured trachea to evaluate the predictions of this model. We found that the steady-state tracheal epithelium is maintained by two progenitor cell pools, secretory and basal cells, and the latter progenitor pool is further divided into two subsets, keratin 14-negative and -positive. After naphthalene-mediated depletion of the secretory and ciliated cell types, the two basal cell pools coordinate to restore the epithelium. Both basal cell types up-regulate keratin 14 and generate a broadly distributed, abundant, and highly mitotic cell pool. Furthermore, basal cell proliferation is associated with generation of differentiated Clara and ciliated cells. The uniform distribution of basal cell progenitors and of their differentiated progeny leads us to propose that the hierarchical organization of tracheal reparative cells be revised to include a facultative basal cell progenitor pool.
Authors:
Brook B Cole; Russell W Smith; Kimberly M Jenkins; Brian B Graham; Paul R Reynolds; Susan D Reynolds
Related Documents :
2337924 - Effects of reversible nare occlusion on the development of the olfactory epithelium in ...
20852734 - Expression of pax6 and sox2 in adult olfactory epithelium.
7622014 - Cortisol induces stress-related changes in the skin of rainbow trout (oncorhynchus myki...
8434644 - Shope papilloma cell and leukocyte proliferation in regressing and progressing lesions.
15342944 - Requirement for neurogenesis to proceed through the division of neuronal progenitors fo...
19308044 - Ngal decreases e-cadherin-mediated cell-cell adhesion and increases cell motility and i...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-06-03
Journal Detail:
Title:  The American journal of pathology     Volume:  177     ISSN:  1525-2191     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-07     Completed Date:  2010-12-06     Revised Date:  2011-08-01    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  362-76     Citation Subset:  AIM; IM    
Affiliation:
Department of Pediatrics, Division of Cell Biology, National Jewish Health, Denver, Colorado, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation / physiology*
Cell Nucleus / ultrastructure
Epithelial Cells / cytology,  drug effects,  physiology*
Female
Gene Expression Profiling
Humans
Keratin-14 / metabolism
Lung Diseases / chemically induced,  pathology
Mice
Naphthalenes / pharmacology
Phenotype
Regeneration
Stem Cells / cytology,  physiology*
Trachea / cytology*,  drug effects,  pathology
Grant Support
ID/Acronym/Agency:
R01HL075585/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Keratin-14; 0/Krt1-14 protein, mouse; 0/Naphthalenes; 91-20-3/naphthalene
Comments/Corrections
Erratum In:
Am J Pathol. 2010 Oct;177(4):2145

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Polyamine transport is mediated by both endocytic and solute carrier transport mechanisms in the gas...
Next Document:  Tumor necrosis factor-induced neutrophil adhesion occurs via sphingosine kinase-1-dependent activati...