Document Detail

Tracheal Basal cells: a facultative progenitor cell pool.
MedLine Citation:
PMID:  20522644     Owner:  NLM     Status:  MEDLINE    
Analysis of lineage relationships in the naphthalene-injured tracheal epithelium demonstrated that two multipotential keratin 14-expressing cells (K14ECs) function as progenitors for Clara and ciliated cells. These K14EC were distinguished by their self-renewal capacity and were hypothesized to reside at the stem and transit amplifying tiers of a tissue-specific stem cell hierarchy. In this study, we used gene expression and histomorphometric analysis of the steady-state and naphthalene-injured trachea to evaluate the predictions of this model. We found that the steady-state tracheal epithelium is maintained by two progenitor cell pools, secretory and basal cells, and the latter progenitor pool is further divided into two subsets, keratin 14-negative and -positive. After naphthalene-mediated depletion of the secretory and ciliated cell types, the two basal cell pools coordinate to restore the epithelium. Both basal cell types up-regulate keratin 14 and generate a broadly distributed, abundant, and highly mitotic cell pool. Furthermore, basal cell proliferation is associated with generation of differentiated Clara and ciliated cells. The uniform distribution of basal cell progenitors and of their differentiated progeny leads us to propose that the hierarchical organization of tracheal reparative cells be revised to include a facultative basal cell progenitor pool.
Brook B Cole; Russell W Smith; Kimberly M Jenkins; Brian B Graham; Paul R Reynolds; Susan D Reynolds
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-06-03
Journal Detail:
Title:  The American journal of pathology     Volume:  177     ISSN:  1525-2191     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-07     Completed Date:  2010-12-06     Revised Date:  2011-08-01    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  362-76     Citation Subset:  AIM; IM    
Department of Pediatrics, Division of Cell Biology, National Jewish Health, Denver, Colorado, USA.
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MeSH Terms
Cell Differentiation / physiology*
Cell Nucleus / ultrastructure
Epithelial Cells / cytology,  drug effects,  physiology*
Gene Expression Profiling
Keratin-14 / metabolism
Lung Diseases / chemically induced,  pathology
Naphthalenes / pharmacology
Stem Cells / cytology,  physiology*
Trachea / cytology*,  drug effects,  pathology
Grant Support
Reg. No./Substance:
0/Keratin-14; 0/Krt1-14 protein, mouse; 0/Naphthalenes; 91-20-3/naphthalene
Erratum In:
Am J Pathol. 2010 Oct;177(4):2145

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