Document Detail


Trace and label-free microRNA detection using oligonucleotide encapsulated silver nanoclusters as probe.
MedLine Citation:
PMID:  22985191     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A simple, sensitive and label-free method for microRNA (miRNA) biosensing was described using oligonucleotide encapsulated silver nanoclusters (Ag-NCs) as effective electrochemical probe. The functional oligonucleotide probe integrates both recognition sequence for hybridization and template sequence for in situ synthesis of Ag-NCs, which appears to possess exceptional metal mimic enzyme property for catalyzing H2O2 reduction. The miRNA assay employs gold electrode to immobilize the molecular beacon (MB) probe. After MB probe subsequently hybridizes with the target and functional probe, the oligonucleotide encapsulated Ag-NCs are brought to the electrode surface and produce a detection signal in response to H2O2 reduction. An electrochemical miRNA biosensor down to 67 fM with a linear range of 5 orders of magnitude was obtained. Meanwhile, the MB probe allows the biosensor to detect the target with high selectivity. The Ag-NCs-based approach provides a novel avenue to detect miRNA with high sensitivity and selectivity while avoiding laborious label and signal amplification. It is convinced that rational introduction of signal amplification strategy to the Ag-NCs-based bioanalysis can further improve the sensitivity. To our best knowledge, this is the first application of the electrocatalytic activity of Ag-NCs in bioanalysis, which would be attractive for genetic analysis and clinic biomedical application.
Authors:
Haifeng Dong; Shi Jin; Huangxian Ju; Kaihong Hao; Liping Xu; Huiting Lu; Xueji Zhang
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-17
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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