| Toxoplasma gondii targets a protein phosphatase 2C to the nuclei of infected host cells. | |
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MedLine Citation:
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PMID: 17085638 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intracellular pathogens have evolved a wide array of mechanisms to invade and co-opt their host cells for intracellular survival. Apicomplexan parasites such as Toxoplasma gondii employ the action of unique secretory organelles named rhoptries for internalization of the parasite and formation of a specialized niche within the host cell. We demonstrate that Toxoplasma gondii also uses secretion from the rhoptries during invasion to deliver a parasite-derived protein phosphatase 2C (PP2C-hn) into the host cell and direct it to the host nucleus. Delivery to the host nucleus does not require completion of invasion, as evidenced by the fact that parasites blocked in the initial stages of invasion with cytochalasin D are able to target PP2C-hn to the host nucleus. We have disrupted the gene encoding PP2C-hn and shown that PP2C-hn-knockout parasites exhibit a mild growth defect that can be rescued by complementation with the wild-type gene. The delivery of parasite effector proteins via the rhoptries provides a novel mechanism for Toxoplasma to directly access the command center of its host cell during infection by the parasite. |
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Authors:
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Luke A Gilbert; Sandeep Ravindran; Jay M Turetzky; John C Boothroyd; Peter J Bradley |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2006-11-03 |
Journal Detail:
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Title: Eukaryotic cell Volume: 6 ISSN: 1535-9778 ISO Abbreviation: Eukaryotic Cell Publication Date: 2007 Jan |
Date Detail:
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Created Date: 2007-01-10 Completed Date: 2007-02-27 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 101130731 Medline TA: Eukaryot Cell Country: United States |
Other Details:
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Languages: eng Pagination: 73-83 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Immunology and Molecular Genetics, University of California--Los Angeles, Los Angeles, CA 90095-1489, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antibodies, Protozoan / immunology Cell Membrane / metabolism Cell Nucleus / enzymology*, parasitology Cells, Cultured Host-Parasite Interactions Humans Metals / chemistry Molecular Sequence Data Phosphoprotein Phosphatases / genetics, metabolism* Protozoan Proteins / immunology, metabolism Sequence Homology, Amino Acid Toxoplasma / genetics, growth & development, immunology, pathogenicity* Toxoplasmosis, Animal |
| Grant Support | |
ID/Acronym/Agency:
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1R01AI064616/AI/NIAID NIH HHS; R01AI21423/AI/NIAID NIH HHS; T32AI07323/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Protozoan; 0/Metals; 0/Protozoan Proteins; EC 3.1.3.16/Phosphoprotein Phosphatases; EC 3.1.3.16/protein phosphatase 2C |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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