Document Detail

Toxoplasma gondii: a family of apical antigens associated with the cytoskeleton.
MedLine Citation:
PMID:  9676707     Owner:  NLM     Status:  MEDLINE    
Antigens associated with the conoid and apical pellicle in Apicomplexan parasites have previously been defined by a panel of monoclonal antibodies that recognize distinct but overlapping protein subsets (Morrissette et al. 1994). To probe the association of these antigens with the apical membrane skeleton of Toxoplasma gondii, we examined their distribution in detergent-extracted tachyzoites and in intracellular parasites treated with drugs that disrupt microtubules. Although intact parasites are labeled only at their extreme apex, immunogold localization in detergent-extracted tachyzoites indicates that the antigens under study are associated with the subpellicular microtubules along their full length. Brief treatment with oryzalin, which results in dissociation of subpellicular microtubules from the pellicle, permits identification of the apical antigens farther along the length of the parasite. Longer dinitroaniline treatment completely eliminates the subpellicular microtubules, after which the apical antigens are found as insoluble patches associated with the parasite pellicle. Taken together, these observations argue that the apical antigens under study are present along the entire microtubule in intact parasites but are inaccessible to antibody binding. In turn, this suggests that these antigens may play a role in the close association of microtubules with the parasite pellicle.
N S Morrissette; D S Roos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental parasitology     Volume:  89     ISSN:  0014-4894     ISO Abbreviation:  Exp. Parasitol.     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1998-07-24     Completed Date:  1998-07-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370713     Medline TA:  Exp Parasitol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  296-303     Citation Subset:  IM    
Department of Biology, University of Pennsylvania, Philadelphia 19104-6018, USA.
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MeSH Terms
Antibodies, Monoclonal / immunology
Antibodies, Protozoan / immunology
Antigens, Protozoan / analysis*,  immunology
Coccidiostats / pharmacology
Cytoskeleton / drug effects,  immunology*
Dinitrobenzenes / pharmacology
Fluorescent Antibody Technique
Frozen Sections
Microscopy, Immunoelectron
Microtubules / drug effects,  immunology
Precipitin Tests
Toxoplasma / drug effects,  immunology*,  ultrastructure
Grant Support
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antibodies, Protozoan; 0/Antigens, Protozoan; 0/Coccidiostats; 0/Dinitrobenzenes; 0/Sulfanilamides; 19044-88-3/oryzalin

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