Document Detail


Toxoplasma co-opts host cells it does not invade.
MedLine Citation:
PMID:  22910631     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Like many intracellular microbes, the protozoan parasite Toxoplasma gondii injects effector proteins into cells it invades. One group of these effector proteins is injected from specialized organelles called the rhoptries, which have previously been described to discharge their contents only during successful invasion of a host cell. In this report, using several reporter systems, we show that in vitro the parasite injects rhoptry proteins into cells it does not productively invade and that the rhoptry effector proteins can manipulate the uninfected cell in a similar manner to infected cells. In addition, as one of the reporter systems uses a rhoptry:Cre recombinase fusion protein, we show that in Cre-reporter mice infected with an encysting Toxoplasma-Cre strain, uninfected-injected cells, which could be derived from aborted invasion or cell-intrinsic killing after invasion, are actually more common than infected-injected cells, especially in the mouse brain, where Toxoplasma encysts and persists. This phenomenon has important implications for how Toxoplasma globally affects its host and opens a new avenue for how other intracellular microbes may similarly manipulate the host environment at large.
Authors:
Anita A Koshy; Hans K Dietrich; David A Christian; Jason H Melehani; Anjali J Shastri; Christopher A Hunter; John C Boothroyd
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-07-26
Journal Detail:
Title:  PLoS pathogens     Volume:  8     ISSN:  1553-7374     ISO Abbreviation:  PLoS Pathog.     Publication Date:  2012  
Date Detail:
Created Date:  2012-08-22     Completed Date:  2013-01-29     Revised Date:  2014-07-02    
Medline Journal Info:
Nlm Unique ID:  101238921     Medline TA:  PLoS Pathog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e1002825     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Fibroblasts / parasitology*
Host-Parasite Interactions*
Humans
Mice
Mice, Inbred C57BL
Protein-Tyrosine Kinases / metabolism*
Protozoan Proteins / metabolism*
STAT6 Transcription Factor / metabolism
Toxoplasma / metabolism*,  pathogenicity*
Grant Support
ID/Acronym/Agency:
AI21423/AI/NIAID NIH HHS; AI41014/AI/NIAID NIH HHS; KO8 NS065116/NS/NINDS NIH HHS; NS069375/NS/NINDS NIH HHS; R01 AI021423/AI/NIAID NIH HHS; R01 AI041014/AI/NIAID NIH HHS; T32 AI007290/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Protozoan Proteins; 0/STAT6 Transcription Factor; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.10.1/Rop16 protein, Toxoplasma gondii
Comments/Corrections

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