Document Detail

Toxins in the skin secretion of the oriental catfish (Plotosus lineatus): immunological properties and immunocytochemical identification of producing cells.
MedLine Citation:
PMID:  3136557     Owner:  NLM     Status:  MEDLINE    
Antiserum against toxin I, one of the lethal factors in the skin secretion from the oriental catfish (Plotosus lineatus), was used to examine immunological properties of the toxic factors and identify toxin-producing cells by an immunocytochemical technique. In immunodiffusion tests, the antiserum formed a precipitin line with toxin I while it formed no precipitin line either with another lethal factor (toxin II) or with a hemolysin. Lethal and edema-forming activities of toxin I were neutralized by the antiserum but lethal activity of toxin II and lytic activity of the hemolysin were not. These results suggested that toxin I can be antigenically distinguished from both toxin II and hemolysin. In immunocytochemical tests using the antiserum, club cells in the epidermis were positively stained, indicating that toxin I is produced in the club cells. Interestingly, venom glandular cells surrounding the dorsal and pectoral spines were also stained. The venom glandular cells appear to produce toxin I or a toxin with the same antigen determinants as toxin I.
K Shiomi; M Takamiya; H Yamanaka; T Kikuchi; Y Suzuki
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  26     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  1988  
Date Detail:
Created Date:  1988-09-19     Completed Date:  1988-09-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  353-61     Citation Subset:  IM    
Department of Food Science and Technology, Tokyo University of Fisheries, Japan.
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MeSH Terms
Catfishes / metabolism*
Chromatography, DEAE-Cellulose
Hemolysis / drug effects
Lethal Dose 50
Skin / cytology,  secretion*
Toxins, Biological / analysis*,  secretion,  toxicity
Reg. No./Substance:
0/Toxins, Biological

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