| Toxicity of sulfonamide-reactive metabolites in HIV-infected, HTLV-infected, and noninfected cells. | |
| | |
MedLine Citation:
|
PMID: 7834397 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
It has been suggested that the high rates of adverse reactions to sulfonamides among patients with AIDS may be related to an increased sensitivity to reactive drug metabolites among HIV-infected cells. To study this hypothesis, we investigated the toxicity of the hydroxylamine of sulfamethoxazole in HIV-infected and noninfected MOLT-3 cultured human T-lymphoblasts. Toxicity was assessed by trypan blue dye exclusion. The hydroxylamine of sulfamethoxazole produced concentration-dependent toxicity in HIV-infected cells, with marked toxicity seen when HIV-infected cells were incubated with 400 microM of the hydroxylamine (82 +/- 8%); this was significantly greater than the toxicity seen among noninfected cells (p < 0.01). There was no concentration-dependent toxicity seen among noninfected cells or in cells infected with HTLV-I, suggesting that the concentration-dependent toxicity seen was specifically related to HIV infection. HIV-infected cells had significantly lower glutathione concentration than did noninfected cells (p < 0.05). Incubation with the hydroxylamine of sulfamethoxazole produced a concentration-dependent decline in glutathione content that was similar in infected and non-infected cells. Co-incubation with glutathione or N-acetylcysteine significantly reduced the toxicity of hydroxylamine of sulfamethoxazole in HIV-infected cells (p < 0.05). Our data supports the role of reactive sulfonamide metabolites in the pathogenesis of adverse reactions to sulfonamides among patients with AIDS. |
| | |
Authors:
|
M J Rieder; R Krause; I A Bird; G A Dekaban |
Related Documents
:
|
8183947 - Inhibition of human immunodeficiency virus infection by agents that interfere with thio... 22329377 - Hepatitis b surface antigen seroconversion is associated with favourable long-term clin... 16289657 - The gammac-cytokine regulated transcription factor, stat5, increases hiv-1 production i... 19645607 - Acetylcholine-esterase inhibitor pyridostigmine decreases t cell overactivation in pati... 18040727 - Urinary interleukin-6 is useful in distinguishing between upper and lower urinary tract... 23771287 - The impact of highly active antiretroviral therapy on high-risk behaviour of hiv-infect... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association Volume: 8 ISSN: 1077-9450 ISO Abbreviation: J. Acquir. Immune Defic. Syndr. Hum. Retrovirol. Publication Date: 1995 Feb |
Date Detail:
|
Created Date: 1995-03-01 Completed Date: 1995-03-01 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9501482 Medline TA: J Acquir Immune Defic Syndr Hum Retrovirol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 134-40 Citation Subset: IM; X |
Affiliation:
|
J. P. Robarts Research Institute, Department of Paediatrics, Western Ontario, London, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Cells, Cultured Deltaretrovirus Infections / drug therapy* Dose-Response Relationship, Drug Drug Hypersensitivity / etiology HIV Infections / drug therapy* Humans Models, Biological Sulfamethoxazole / analogs & derivatives*, toxicity T-Lymphocytes / drug effects*, virology Toxicity Tests |
| Chemical | |
Reg. No./Substance:
|
114438-33-4/sulfamethoxazole hydroxylamine; 723-46-6/Sulfamethoxazole |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Biological and genetic changes in ovine lentivirus strains following passage in isogeneic twin lambs...
Next Document: Combination therapy with recombinant human soluble CD4-immunoglobulin G and zidovudine in patients w...