Document Detail


Toxicity of polyhexanide and hydrogen peroxide on human chondrocytes in vitro.
MedLine Citation:
PMID:  21717992     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The treatment of acute joint infections has an important impact on long-term outcome and remains an unsolved problem. The most frequent bacteria are staphylococci, streptococci, and gram-negative bacteria. In septic surgery, polyhexanide and hydrogen peroxide are the most frequently used local antiseptics. The aim of this study was to examine the hypothesis that antiseptics induce cell death of human chondrocytes after a short incubation time.Human chondrocytes were treated with different concentrations of polyhexanide and hydrogen peroxide. Toxicity analysis was determined by visualization of cell structure using light microscopy, lactate dehydrogenase release, and determination of living and total cell numbers after addition of polyhexanide and hydrogen peroxide. Light microscopic data revealed a defect cell structure after addition of both antiseptics. Lactate dehydrogenase activity showed a significant increase of enzyme expression after a short incubation with polyhexanide. The determination of vital chondrocytes showed a significant decrease of vital and total cell numbers after addition with polyhexanide and hydrogen peroxide.Both antiseptic solutions induce significant cell death of human chondrocytes after a short incubation time. Polyhexanide possibly has more toxic potential than hydrogen peroxide against human chondrocytes after an application >15 minutes. Therefore, both substances should only be applied for a short time (<15 minutes) and the joint irrigated to wash out the antiseptic substance prior to wound closure.
Authors:
Eric Röhner; Joern B Seeger; Paula Hoff; Stephanie Dähn-Wollenberg; Carsten Perka; Georg Matziolis
Publication Detail:
Type:  Journal Article     Date:  2011-07-07
Journal Detail:
Title:  Orthopedics     Volume:  34     ISSN:  1938-2367     ISO Abbreviation:  Orthopedics     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-07-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7806107     Medline TA:  Orthopedics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e290-4     Citation Subset:  IM    
Copyright Information:
Copyright 2011, SLACK Incorporated.
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