Document Detail

Toxicity of metal oxide nanoparticles in mammalian cells.
MedLine Citation:
PMID:  17114101     Owner:  NLM     Status:  MEDLINE    
Along with existing and emerging use of nanoscale materials, growing concerns have arisen about their unintentional health and environmental impact. The objective of the ongoing study was to assess the toxicity profile of metal oxide nanoparticles proposed for use in industrial production methodology. Metal oxide nanoparticles used in this study included TiO2, ZnO, Fe3O4, Al2O3, and CrO3 with particle sizes ranging from 30 to 45 nm. Cellular morphology, mitochondrial function, membrane leakage of lactate dehydrogenase (LDH), permeability of plasma membrane, and apoptosis were assessed under controlled and exposed conditions (2 to 72 h of exposure). The microscopic studies demonstrated that nanoparticle-exposed Neuro-2A cells (especially ZnO) at doses >100 microg/mL became abnormal in size, displaying cellular shrinkage, and detachment from the surface of flasks. Mitochondrial function decreased significantly in the cells exposed to ZnO at 50 to 100 microg/mL. However, Fe3O4, Al2O3, and TiO2 had no measurable effect on the cells until the concentrations reached greater than 200 microg/mL. LDH leakage significantly increased in the cells exposed to ZnO (50 to 100 microg/mL), while other nanoparticles tested displayed LDH leakage only at higher doses (>200 microg/mL). Flow cytometer tests showed that apoptosis took place in cells exposed to ZnO nanoparticles. More cells became necrotic as the concentrations increased.
Hueiwang Anna Jeng; James Swanson
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering     Volume:  41     ISSN:  1093-4529     ISO Abbreviation:  J Environ Sci Health A Tox Hazard Subst Environ Eng     Publication Date:  2006  
Date Detail:
Created Date:  2006-11-20     Completed Date:  2007-02-28     Revised Date:  2009-08-14    
Medline Journal Info:
Nlm Unique ID:  9812551     Medline TA:  J Environ Sci Health A Tox Hazard Subst Environ Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2699-711     Citation Subset:  IM    
Department of Community and Environmental Health, College of Health Sciences, Old Dominion University, Norfolk, Virginia 23529, USA.
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MeSH Terms
Cell Line
Cell Membrane Permeability
Cell Shape
Metals, Heavy / toxicity*
Mitochondria / physiology
Oxides / toxicity*
Reg. No./Substance:
0/Metals, Heavy; 0/Oxides

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