Document Detail

Toxicity of cadmium to rat osteosarcoma cells (ROS 17/2.8): protective effect of 1 alpha,25-dihydroxyvitamin D3.
MedLine Citation:
PMID:  2315924     Owner:  NLM     Status:  MEDLINE    
Inadequate vitamin D intake is an important cofactor in clinical and experimental bone disease induced by chronic cadmium exposure. The interaction was investigated by culture of rat osteoblastic osteosarcoma cells (ROS 17/2.8) in a serum-free medium with equimolar concentrations of cadmium chloride and 1 alpha,25-(OH)2 vitamin D3. After addition of cadmium alone to culture medium, the unstimulated secretion of osteocalcin and cellular alkaline phosphatase activity were inhibited at 10 pM, and of DNA synthesis and proline incorporation into collagen at 500 nM. In the presence of equimolar amounts of cadmium and 1 alpha,25-(OH)2 vitamin D3, all four responses paralleled those of 1 alpha,25-(OH)2 vitamin D3 alone up to the inhibitory concentration of 500 nM cadmium. Neither 10 nM 1 alpha,25-(OH)2 vitamin D3 nor 1 microM cadmium induced synthesis of metallothionein in these cells indicating that the protective effect of D3 was not related to the induction of a metallothionein-like protein in ROS 17/2.8 cells. In the presence or absence of D3, cadmium inhibited osteoblastic function at concentrations below the whole-organ concentration of cadmium in bone as reported in experimental and clinical cadmium-induced osteotoxicity. The extreme sensitivity of ROS 17/2.8 cells to cadmium may relate to the absence of metallothionein synthesis.
C R Angle; D J Thomas; S A Swanson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Toxicology and applied pharmacology     Volume:  103     ISSN:  0041-008X     ISO Abbreviation:  Toxicol. Appl. Pharmacol.     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1990-04-23     Completed Date:  1990-04-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0416575     Medline TA:  Toxicol Appl Pharmacol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  113-20     Citation Subset:  IM    
Department of Pediatrics, University of Nebraska Medical Center, Omaha 68105.
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MeSH Terms
Cadmium / toxicity*
Calcitriol / pharmacology*
DNA / analysis
Metallothionein / biosynthesis
Osteoblasts / drug effects*,  metabolism
Osteocalcin / secretion
Osteosarcoma / pathology
Tumor Cells, Cultured / drug effects
Grant Support
Reg. No./Substance:
104982-03-8/Osteocalcin; 32222-06-3/Calcitriol; 7440-43-9/Cadmium; 9007-49-2/DNA; 9038-94-2/Metallothionein

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