Document Detail

Toxicity, binding and internalization of the pea-A1b entomotoxin in Sf9 cells.
MedLine Citation:
PMID:  17845830     Owner:  NLM     Status:  MEDLINE    
PA1b (Pea Albumin 1b) is a peptide toxin lethal for certain insects. This paper shows that the cultured insect cells Sf9 are sensitive to the toxin and display a high-affinity binding site for PA1b. Mammalian cells are not sensitive and no binding activity was detected. Signs of apoptosis of the Sf9 cells were observed in response to the toxin. The use of this cellular model also demonstrated that PA1b was internalized in the cells, via the binding site, raising the new question of the role of this toxin within the cell, and of the mechanisms leading to cell death.
Isabelle Rahioui; Christian Laugier; Séverine Balmand; Pedro Da Silva; Yvan Rahbe; Frédéric Gressent
Publication Detail:
Type:  Journal Article     Date:  2007-08-06
Journal Detail:
Title:  Biochimie     Volume:  89     ISSN:  0300-9084     ISO Abbreviation:  Biochimie     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-26     Completed Date:  2008-04-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1264604     Medline TA:  Biochimie     Country:  France    
Other Details:
Languages:  eng     Pagination:  1539-43     Citation Subset:  IM    
UMR203 Biologie Fonctionnelle Insectes et Interactions, IFR41, INRA, INSA de Lyon, INSA Bâtiment Louis Pasteur, F-69100 Villeurbanne Cedex, France.
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MeSH Terms
Albumins / chemistry*
Apoptosis / drug effects
Binding Sites
Cell Culture Techniques
Cell Survival / drug effects
Cells, Cultured
Endotoxins / chemistry*,  isolation & purification,  pharmacology
Iodine Radioisotopes / metabolism
Molecular Weight
Peas / metabolism*
Plant Proteins / chemistry
Protein Binding
Protein Isoforms / chemistry
Spodoptera / cytology*,  metabolism*,  ultrastructure
Time Factors
Reg. No./Substance:
0/Albumins; 0/Endotoxins; 0/Iodine Radioisotopes; 0/Plant Proteins; 0/Protein Isoforms

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