| Toxicity, binding and internalization of the pea-A1b entomotoxin in Sf9 cells. | |
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MedLine Citation:
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PMID: 17845830 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PA1b (Pea Albumin 1b) is a peptide toxin lethal for certain insects. This paper shows that the cultured insect cells Sf9 are sensitive to the toxin and display a high-affinity binding site for PA1b. Mammalian cells are not sensitive and no binding activity was detected. Signs of apoptosis of the Sf9 cells were observed in response to the toxin. The use of this cellular model also demonstrated that PA1b was internalized in the cells, via the binding site, raising the new question of the role of this toxin within the cell, and of the mechanisms leading to cell death. |
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Authors:
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Isabelle Rahioui; Christian Laugier; Séverine Balmand; Pedro Da Silva; Yvan Rahbe; Frédéric Gressent |
Publication Detail:
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Type: Journal Article Date: 2007-08-06 |
Journal Detail:
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Title: Biochimie Volume: 89 ISSN: 0300-9084 ISO Abbreviation: Biochimie Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-11-26 Completed Date: 2008-04-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1264604 Medline TA: Biochimie Country: France |
Other Details:
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Languages: eng Pagination: 1539-43 Citation Subset: IM |
Affiliation:
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UMR203 Biologie Fonctionnelle Insectes et Interactions, IFR41, INRA, INSA de Lyon, INSA Bâtiment Louis Pasteur, F-69100 Villeurbanne Cedex, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Albumins
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chemistry* Animals Apoptosis / drug effects Binding Sites Cell Culture Techniques Cell Survival / drug effects Cells, Cultured Endotoxins / chemistry*, isolation & purification, pharmacology Iodine Radioisotopes / metabolism Kinetics Molecular Weight Peas / metabolism* Plant Proteins / chemistry Protein Binding Protein Isoforms / chemistry Spodoptera / cytology*, metabolism*, ultrastructure Temperature Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Albumins; 0/Endotoxins; 0/Iodine Radioisotopes; 0/Plant Proteins; 0/Protein Isoforms |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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