Document Detail

Toxicity assessment of extracts from infusion sets in cEND brain endothelial cells.
MedLine Citation:
PMID:  22643027     Owner:  NLM     Status:  Publisher    
In vitro safety assessment of disposable medical devices, including infusion sets, is usually performed using L-929 mouse keratinocytes. However, cells of different origin (endothelial, lymphoid and myeloid cells) are also exposed to infusion sets' extractables during their clinical use. We studied whether the cEND mouse brain endothelial cells can be suitable for in vitro safety assessment of infusion sets. We analyzed infusion sets from different manufacturers that varied in design and storage time. cEND cells were incubated with extracts of individual parts of the infusion sets (tube, cup, latex), and relative toxicities were analyzed using MTT test, DCFH-DA-based analysis of reactive oxygen species formation, apoptosis and cell cycle analyses. We identified a pattern of yellowing of the infusion sets upon storage and revealed that it originated from the latex part. Extracts of the individual parts of the infusion sets, primarily of the latex, were toxic to the cEND cells leading to induction of apoptosis and cell death. We conclude that infusion sets release extractables that can be toxic to the endothelial cells of the patients that receive infusion. We suggest to use cEND cells for in vitro safety assessment of infusion sets and other medical devices that release extractables to the bloodstream.
George R Dakwar; Veronika Kaplun; Lena Kojukarov; Pavel Gorenbein; Ilana Schumacher; Diana Kontorovich; Carola Förster; Elie Beit-Yannai; David Stepensky
Related Documents :
20811567 - Inherited adaptation of genome-rewired cells in response to a challenging environment.
6744127 - A transport-dependent energy burden imposed by growth of enterobacter cloacae in the pr...
12566067 - Icodextrin-induced lipid peroxidation disrupts the mesothelial cell cycle engine.
19223597 - Degradation of camp-responsive element-binding protein by the ubiquitin-proteasome path...
6039377 - Cell junctions in ommatidia of limulus.
8180777 - Immunogold analysis of antioxidant enzymes in human renal cell carcinoma.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-19
Journal Detail:
Title:  International journal of pharmaceutics     Volume:  -     ISSN:  1873-3476     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7804127     Medline TA:  Int J Pharm     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Department of Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Functional region prediction with a set of appropriate homologous sequences--an index for sequence s...
Next Document:  Handwriting: 3-D Kinetic Synergies in Circle Drawing Movements.