Document Detail

Toxicity of Streptococcus pneumoniae in neurons, astrocytes, and microglia in vitro.
MedLine Citation:
PMID:  7751718     Owner:  NLM     Status:  MEDLINE    
The toxicity of pneumococci and endotoxin in primary cultures of rat neurons, astrocytes, and microglia and in a human astrocyte and two human glial cell lines was determined. Heat-inactivated, rough pneumococci (up to 10(8) cfu/mL) or their cell wall (up to 50 micrograms/mL) produced dose-dependent toxicity after 48 h in microglial cells and to a lesser extent in astrocytes but not in neurons. Toxicity was similar for equivalent doses of heat-inactivated organisms and pneumococcal cell wall, but time-course experiments showed significant differences between the two stimuli. Endotoxin at concentrations of up to 5 micrograms/mL did not induce significant toxicity in any of the cells. Thus, pneumococci can induce toxicity in two brain cell types, microglia and astrocytes, and the pneumococcal cell wall appears to mediate toxicity. Direct toxic effects of bacteria on brain cells may in part be responsible for brain injury during meningitis.
Y S Kim; S Kennedy; M G Täuber
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of infectious diseases     Volume:  171     ISSN:  0022-1899     ISO Abbreviation:  J. Infect. Dis.     Publication Date:  1995 May 
Date Detail:
Created Date:  1995-06-22     Completed Date:  1995-06-22     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0413675     Medline TA:  J Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1363-8     Citation Subset:  AIM; IM    
Infectious Diseases Laboratory, San Francisco General Hospital, CA, USA.
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MeSH Terms
Astrocytes / cytology*
Bacterial Toxins / toxicity*
Cell Death
Cell Survival
Cell Wall / chemistry
Cells, Cultured
Endotoxins / toxicity*
Hot Temperature
Lipopolysaccharides / toxicity
Microglia / cytology*
Neurons / cytology*
Rats, Sprague-Dawley
Streptococcus pneumoniae / chemistry*
Reg. No./Substance:
0/Bacterial Toxins; 0/Endotoxins; 0/Lipopolysaccharides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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