| Toxicarioside A, isolated from tropical Antiaris toxicaria, blocks endoglin/TGF-β signaling in a bone marrow stromal cell line. | |
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MedLine Citation:
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PMID: 22221748 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate possible mechanism of toxicarioside A in HS-5 bone stromal cells. METHODS: HS-5 bone stromal cells were cultured in media supplemented with various concentrations of toxicarioside A or control DMSO (not treatment). Endoglin and TGF-β were detected by Northern and Western blot analysis and quantified in a standard method. Downstream molecules of endoglin and TGF-β (Smad1, Smad2 and their active phosphorylated counterparts, pSmad1 and pSmad2) were also detected and quantified by Western blot analysis. In addition, cell proliferation assay and small interfering RNA (siRNA) against endoglin were used to certificate the function of endolgin in the HS-5 cells. RESULTS: Compared with the not treated (0 μg/mL) or DMSO treated control HS-5 cells, HS-5 cells treated with toxicarioside A were found significant attenuation of endolgin and TGF-β expression. Significant inhibition of cell proliferation was also found in the HS-5 cells treated with toxicarioside A. ALK1-related Smad1 and ALK5-related Smad2 were decreased in HS-5 cells treated with toxicarioside A. In addition, phosphorylated Smad1 (pSmad1) and Smad2 (pSmad2) were also found attenuation in toxicarioside A-treated HS-5 cells. RNA interference showed that blockage of endoglin by siRNA also decreased Smad1 and Smad2 expression in HS-5 cells. CONCLUSIONS: Our results indicate that toxicarioside A can influence bone marrow stromal HS-5's function and inhibit HS-5 cell proliferation by alteration of endoglin-related ALK1 (Smad1) and ALK5 (Smad2) signaling. |
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Authors:
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Yue-Nan Li; Feng-Ying Huang; Wen-Li Mei; Hao-Fu Dai; Jun-Li Guo; Guang-Hong Tan; Peng Zhou |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Asian Pacific journal of tropical medicine Volume: 5 ISSN: 1995-7645 ISO Abbreviation: Asian Pac J Trop Med Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-01-06 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101533720 Medline TA: Asian Pac J Trop Med Country: Singapore |
Other Details:
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Languages: eng Pagination: 91-7 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Hainan Medical College. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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Agriculture College, Hainan University, Haikou, 570228, China; Hainan Provincial Key Laboratory of Tropical Medicine, Hainan Medical College, Haikou 571101, China; Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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