Document Detail


Toxic neurofilamentous axonopathies and fast axonal transport. V. Reduced bidirectional vesicle transport in cultured neurons by acrylamide and glycidamide.
MedLine Citation:
PMID:  7517455     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fast axonal transport deficiencies as mechanisms of action of acrylamide in producing axonal degeneration are under evaluation. The current study determines the effects of acrylamide and several analogues on the number of vesicles moving within the neurite processes of cultured rat embryonic neurons. Acrylamide produced severe, concentration-dependent (0.25-1.0 mM) and time-dependent (0-60 min) reduction in the quantity of vesicles translocated in both the anterograde and retrograde directions. Glycidamide, a potential neurotoxic metabolite of acrylamide, produced a time-dependent but not a concentration-dependent (in the 0.25-1.0 mM range) reduction in bidirectional transport. Based on inhibition at 60 min, glycidamide was estimated to be 4 times more potent than acrylamide in altering transport. Propionamide, a C1-C2 saturated nonneurotoxic acrylamide analogue, had no effect on axonal transport. While a tendency for methylene bisacrylamide (MbACR) to reduce vesicle transport was noted, at the concentration used no statistically significant differences from control were observed. The data support the correlation between toxicant-induced fast anterograde and retrograde axonal transport reductions and axonal degeneration produced by acrylamide and its analogues.
Authors:
C H Harris; A K Gulati; M A Friedman; D W Sickles
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of toxicology and environmental health     Volume:  42     ISSN:  0098-4108     ISO Abbreviation:  J Toxicol Environ Health     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-08-04     Completed Date:  1994-08-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7513622     Medline TA:  J Toxicol Environ Health     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  343-56     Citation Subset:  IM    
Affiliation:
Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta 30912-2000.
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MeSH Terms
Descriptor/Qualifier:
Acrylamide
Acrylamides / toxicity*
Analysis of Variance
Animals
Axonal Transport / drug effects*
Cells, Cultured
Dose-Response Relationship, Drug
Epoxy Compounds / toxicity*
Rats
Rats, Sprague-Dawley
Time Factors
Chemical
Reg. No./Substance:
0/Acrylamides; 0/Epoxy Compounds; 5694-00-8/glycidamide; 79-06-1/Acrylamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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