| Toxic effects following phosgene exposure of human epithelial lung cells in vitro using a CULTEX® system. | |
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MedLine Citation:
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PMID: 21945045 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The aim of the present study was to investigate toxic effects following phosgene exposure of human epithelial lung cells (A549) in vitro using a CULTEX® system. In particular, toxic effects regarding early biomarkers emerging during the latency period following exposure might be of great value for medical treatment. Cells cultured on semi-permeable membranes were directly exposed at the liquid-air interface to different concentrations of phosgene, or dry medical air. Cell membrane integrity (leakage of LDH), metabolic activity (reduction of Alamar Blue), oxidative damage (GSH, and HO-1, in cell lysates), and release of IL-8, were studied. For most of the above-mentioned biological end-point markers, significant changes could be assessed following a 20min exposure to 1.0ppm and 2.0ppm phosgene. Moreover, except for IL-8, all biological marker profiles showed to be in line with results obtained by others in animal studies. The C×t value of 40ppmmin appeared to be constant. The overall results suggest that at 4h post-exposure a maximal level of toxicity was achieved. Our results demonstrate the suitability of a CULTEX® system to detect toxic effects induced by phosgene on human epithelial lung cells, which may contribute to the discovery of early biomarkers for new medical countermeasures. |
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Authors:
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Dorien Wijte; Marcel J Alblas; Daan Noort; Jan P Langenberg; Herman P M van Helden |
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Publication Detail:
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Type: - Date: 2011-9-12 |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: - ISSN: 1879-3177 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Ltd. |
Affiliation:
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TNO Defence, Security and Safety, Business Unit Biological and Chemical Protection, P.O. Box 45, 2280 AA Rijswijk, The Netherlands. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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