| Toxic effect of L-2-chloropropionate on cultured rat cerebellar granule cells is ameliorated after inhibition of reactive oxygen species formation. | |
| | |
MedLine Citation:
|
PMID: 11746428 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Oral administration of rats to L-2-chloropropionate (L-CPA) causes selective necrosis to the granule cell layer of the cerebellum in vivo and to cultured rat cerebellar granule cells in vitro. The present study was conducted to characterize the involvement of reactive oxygen species (ROS) in cell death of L-CPA to rat cerebellar granule cells in vitro. Exposure to L-CPA (0.625-10 mM) produced a concentration dependent increase in formation of 2,7-dichlorofluorescein (DCF) as a measure of formation of ROS. The elevation of ROS was inhibited after incubation of the cells with the ERK-type of MAP kinases inhibitor U0126, the mitochondrial permeability transition pore inhibitor cyclosporin A (CSA), the antioxidant vitamin E, and the spin trap N-tert-butyl-alpha-(2-sulfophenyl)-nitrone (S-PBN). Measurements of nitrite (NO(2)) in the cell culture supernatant using the Griess reagent indicate generation of nitric oxide (NO) after exposure to L-CPA. Incubation with L-CPA (10 mM) for 48 hr lead to cell death (90%). When the granule cells were incubated with L-CPA in combination with the inhibitors of free radical production, the cell death was ameliorated. The results show that L-CPA is toxic to granular cells by production of ROS. |
| | |
Authors:
|
O Myhre; B Bjugan; F Fonnum |
Related Documents
:
|
9361128 - Flow cytometry study of the role of superoxide anion and hydrogen peroxide in cellular ... 7920608 - Solar ultraviolet radiation induces enhanced accumulation of oxygen radicals in murine ... 19339548 - Bax inhibitor 1 regulates er-stress-induced ros accumulation through the regulation of ... 3963208 - Increases of cell atp produced by exogenous adenine nucleotides in isolated rabbit kidn... 8054538 - Biosynthesis and processing of the glycosylphosphatidylinositol anchor in mammalian cells. 22381678 - Extra cytoplasmic function σ factor activation. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of neuroscience research Volume: 66 ISSN: 0360-4012 ISO Abbreviation: J. Neurosci. Res. Publication Date: 2001 Dec |
Date Detail:
|
Created Date: 2001-12-17 Completed Date: 2002-02-01 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 7600111 Medline TA: J Neurosci Res Country: United States |
Other Details:
|
Languages: eng Pagination: 992-7 Citation Subset: IM |
Copyright Information:
|
Copyright 2001 Wiley-Liss, Inc. |
Affiliation:
|
Norwegian Defence Research Establishment, Division for Protection and Materiel, PO Box 25, N-2027 Kjeller, Norway. oddvar.myhre@ffi.no |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Animals, Newborn Antioxidants / pharmacology Cell Death / drug effects, physiology* Cells, Cultured Cerebellar Cortex / drug effects*, metabolism, physiopathology Enzyme Inhibitors / pharmacology Glutathione / drug effects, metabolism Immunosuppressive Agents / pharmacology Intracellular Membranes / drug effects, metabolism Mitochondria / drug effects, metabolism Mitogen-Activated Protein Kinases / antagonists & inhibitors, metabolism Neurons / drug effects*, metabolism Neuroprotective Agents / pharmacology Neurotoxins / toxicity* Oxidative Stress / drug effects, physiology* Permeability / drug effects Propionic Acids / toxicity* Rats Rats, Wistar Reactive Oxygen Species / antagonists & inhibitors*, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Antioxidants; 0/Enzyme Inhibitors; 0/Immunosuppressive Agents; 0/Neuroprotective Agents; 0/Neurotoxins; 0/Propionic Acids; 0/Reactive Oxygen Species; 598-78-7/2-chloropropionic acid; 70-18-8/Glutathione; EC 2.7.11.24/Mitogen-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Ammonia induces the mitochondrial permeability transition in primary cultures of rat astrocytes.
Next Document: Identification and characterization of uptake systems for cystine and cysteine in cultured astrocyte...