Document Detail


Toxic and biochemical effects of zinc in Caco-2 cells.
MedLine Citation:
PMID:  14568236     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Zinc (in relatively high concentrations) can be toxic to intestinal cells. The aim of the present study was to quanitfy cellular injury in preconfluent, colonic cancerous cells and in postconfluent, differentiating human intestinal Caco-2 cells. Cellular damage was measured by using cell proliferation, lactate dehydrogenase (LDH)-release, and apoptosis studies. Furthermore, the activities of the major antioxidative enzymes [superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase] and differentiation markers (alkaline phosphatase and aminopeptidase-N) were determined after exposure of the cells to increasing amounts of zinc sulfate. Proliferation and viability decreased in a concentration-dependent manner. A noticeable increase of LDH-release correlated to cell rounding and detachment at relatively high zinc levels (200 muM) was observed in both groups of cells. Above 100 muM of zinc, significant apoptotic activity was found in the preconfluent cells. Zinc supplementation did not alter SOD activities. However, GPx and, in part, catalase activities tended to be higher in zinc-treated cells (nevertheless the results were not significant). Differentiation markers were noticeably induced by increasing amounts of zinc, especially in the preconfluent cells. In conclusion, we suggest that the susceptibility to zinc induced damage is equal in both confluentation groups of Caco-2 cells. Risk assessment for high concentrations seems recommendable.
Authors:
Bettina Zödl; Michaela Zeiner; Mansour Sargazi; Norman B Roberts; Wolfgang Marktl; Ilse Steffan; Cem Ekmekcioglu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of inorganic biochemistry     Volume:  97     ISSN:  0162-0134     ISO Abbreviation:  J. Inorg. Biochem.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-10-21     Completed Date:  2004-02-04     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7905788     Medline TA:  J Inorg Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  324-30     Citation Subset:  IM    
Affiliation:
Department of Physiology, Faculty of Medicine, University of Vienna, Schwarzspanierstrasse 17, A-1090, Vienna, Austria. bettina.zoedl@univie.ac.at
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / drug effects,  metabolism
Antigens, CD13 / drug effects,  metabolism
Apoptosis / drug effects,  physiology
Biological Markers / analysis
Caco-2 Cells
Catalase / drug effects,  metabolism
Cell Differentiation / drug effects,  physiology
Cell Division / drug effects,  physiology
Dose-Response Relationship, Drug
Epithelial Cells / drug effects,  physiology
Glutathione Peroxidase / drug effects,  metabolism
Humans
Intestines / drug effects*,  metabolism
L-Lactate Dehydrogenase / drug effects,  metabolism
Superoxide Dismutase / drug effects,  metabolism
Zinc / metabolism*,  pharmacology*,  toxicity
Chemical
Reg. No./Substance:
0/Biological Markers; 7440-66-6/Zinc; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.11.1.6/Catalase; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase; EC 3.1.3.1/Alkaline Phosphatase; EC 3.4.11.2/Antigens, CD13

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