Document Detail


Toxic activity of the CdtB component of Haemophilus ducreyi cytolethal distending toxin expressed from an adenovirus 5 vector.
MedLine Citation:
PMID:  20132178     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Haemophilus ducreyi cytolethal distending toxin (HdCDT) catalytic subunit CdtB has DNase-like activity and mediates DNA damage after its delivery into target cells. We constructed a replication-deficient adenovirus type 5 (Ad5) vector expressing CdtB and investigated the toxic properties of this vector on HeLa cells. Ad5CdtB caused loss of cell viability, morphologic changes, and cell cycle arrest, findings similar to HdCDT intoxication. This confirmed that CdtB is responsible for the toxicity of the holotoxin when expressed in cells following transduction by an adenoviral vector, and indicated a possible potential of this novel strategy in studies of activity of intracellular products and in gene therapy of cancer.
Authors:
Catharina Wising; Maria Magnusson; Karin Ahlman; Leif Lindholm; Teresa Lagerg?rd
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  APMIS : acta pathologica, microbiologica, et immunologica Scandinavica     Volume:  118     ISSN:  1600-0463     ISO Abbreviation:  APMIS     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-05     Completed Date:  2010-03-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8803400     Medline TA:  APMIS     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  143-9     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, Institute of Biomedicine, The Sahlgrenska Academy, G?teborg University, G?teborg, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics*
Bacterial Toxins / genetics,  toxicity*
Genetic Vectors*
Haemophilus ducreyi / pathogenicity*
Hela Cells
Humans
Chemical
Reg. No./Substance:
0/Bacterial Toxins; 0/cytolethal distending toxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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