Document Detail

Toxic action of etoposide on mouse peritoneal macrophages and its modulation by interleukin 3.
MedLine Citation:
PMID:  14695654     Owner:  NLM     Status:  MEDLINE    
In the present work, we have studied the toxic action of etoposide on mouse peritoneal macrophages. First, we have determined the induction of DNA fragmentation by this antitumour compound. To study the possible influence of interleukin 3 on the effects of etoposide on mouse macrophages, we studied intracellular protein phosphorylation induced by interleukin 3. After incubation of the cells in the presence of interleukin 3, increased phosphorylation levels of proteins of estimated molecular weights of around 29,000, 34,000, 50,000 and 61,000 daltons were observed. We have also investigated a possible influence of interleukin 3 on DNA degradation induced by etoposide. The changes of Bax levels induced by etoposide that we have observed seemed to be modulated by this cytokine. From these results, a possible role of interleukin 3 can be suggested in the attenuation of some toxic effects produced by etoposide in mouse peritoneal macrophages. Thus, a therapeutic application of interleukin 3 on antitumour treatments in cells from the mononuclear phagocytic system might be proposed.
Gemma Olmos; L Alfredo Lotero; Angel Herráez; José C Diez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biochemistry and function     Volume:  22     ISSN:  0263-6484     ISO Abbreviation:  Cell Biochem. Funct.     Publication Date:    2004 Jan-Feb
Date Detail:
Created Date:  2003-12-25     Completed Date:  2004-08-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8305874     Medline TA:  Cell Biochem Funct     Country:  England    
Other Details:
Languages:  eng     Pagination:  45-52     Citation Subset:  IM    
Copyright Information:
Copyright 2003 John Wiley & Sons, Ltd.
Departamento de Bioquímica y Biología Molecular, Campus Universitario, Universidad de Alcalá, Madrid, Spain.
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MeSH Terms
Blotting, Western
Cells, Cultured
DNA Fragmentation / drug effects*
Etoposide / toxicity*
Interleukin-3 / pharmacology*
Macrophages, Peritoneal / drug effects*,  metabolism
Nitric Oxide / metabolism
Phosphorylation / drug effects
Protein Biosynthesis*
Proto-Oncogene Proteins / biosynthesis
Proto-Oncogene Proteins c-bcl-2*
Tumor Suppressor Protein p53 / biosynthesis
bcl-2-Associated X Protein
Reg. No./Substance:
0/Bax protein, mouse; 0/Interleukin-3; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Tumor Suppressor Protein p53; 0/bcl-2-Associated X Protein; 10102-43-9/Nitric Oxide; 33419-42-0/Etoposide

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