| Towards targeted mutagenesis and gene replacement in plants. | |
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MedLine Citation:
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PMID: 16243407 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Advances in the development of biotechnological tools for plant gene disruption and repair have lagged behind the rapid progress made in whole-genome sequencing of many model and crop plant species. Plant DNA-repair machinery predominantly uses non-homologous end-joining (NHEJ), making the homologous recombination (HR)-based methods, which have proved fruitful for gene targeting in non-plant systems, unsuitable for use in plant systems. Two recent reports describe successful targeted mutagenesis and gene targeting in Arabidopsis by either harnessing the plant NHEJ machinery using site-specific induction of double-strand breaks (DSBs), or by activation of a HR pathway through overexpression of a yeast DNA recombination gene in transgenic plants. These reports provide a foundation from which new technologies for site-specific genome alterations in plant species can be developed. |
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Authors:
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Tzvi Tzfira; Charles White |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Review Date: 2005-10-21 |
Journal Detail:
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Title: Trends in biotechnology Volume: 23 ISSN: 0167-7799 ISO Abbreviation: Trends Biotechnol. Publication Date: 2005 Dec |
Date Detail:
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Created Date: 2005-11-29 Completed Date: 2006-01-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8310903 Medline TA: Trends Biotechnol Country: England |
Other Details:
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Languages: eng Pagination: 567-9 Citation Subset: IM |
Affiliation:
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Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA. ttzfira@umich.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Arabidopsis
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genetics DNA Deoxyribonucleases / genetics, metabolism Genetic Engineering / methods* Mutagenesis* Plants, Genetically Modified* Recombination, Genetic Zinc Fingers |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA; EC 3.1.-/Deoxyribonucleases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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