Document Detail


Towards a synthetic chloroplast.
MedLine Citation:
PMID:  21533097     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The evolution of eukaryotic cells is widely agreed to have proceeded through a series of endosymbiotic events between larger cells and proteobacteria or cyanobacteria, leading to the formation of mitochondria or chloroplasts, respectively. Engineered endosymbiotic relationships between different species of cells are a valuable tool for synthetic biology, where engineered pathways based on two species could take advantage of the unique abilities of each mutualistic partner.
RESULTS: We explored the possibility of using the photosynthetic bacterium Synechococcus elongatus PCC 7942 as a platform for studying evolutionary dynamics and for designing two-species synthetic biological systems. We observed that the cyanobacteria were relatively harmless to eukaryotic host cells compared to Escherichia coli when injected into the embryos of zebrafish, Danio rerio, or taken up by mammalian macrophages. In addition, when engineered with invasin from Yersinia pestis and listeriolysin O from Listeria monocytogenes, S. elongatus was able to invade cultured mammalian cells and divide inside macrophages.
CONCLUSION: Our results show that it is possible to engineer photosynthetic bacteria to invade the cytoplasm of mammalian cells for further engineering and applications in synthetic biology. Engineered invasive but non-pathogenic or immunogenic photosynthetic bacteria have great potential as synthetic biological devices.
Authors:
Christina M Agapakis; Henrike Niederholtmeyer; Ramil R Noche; Tami D Lieberman; Sean G Megason; Jeffrey C Way; Pamela A Silver
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-04-20
Journal Detail:
Title:  PloS one     Volume:  6     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2011  
Date Detail:
Created Date:  2011-05-02     Completed Date:  2011-08-30     Revised Date:  2014-04-23    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e18877     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / genetics
Base Sequence
Chloroplasts*
DNA / genetics
DNA Primers
Evolution, Molecular
Genetic Engineering
Macrophages / microbiology
Photosynthesis
Plasmids
Synechococcus / genetics,  growth & development,  physiology*
Zebrafish / embryology
Grant Support
ID/Acronym/Agency:
T32 GM080177/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA Primers; 9007-49-2/DNA
Comments/Corrections

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