Document Detail


Towards the production of high levels of eicosapentaenoic acid in transgenic plants: the effects of different host species, genes and promoters.
MedLine Citation:
PMID:  19582587     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Eicosapentaenoic acid (EPA, 20:5n-3) plays an important role in many aspects of human health. In our efforts towards producing high levels of EPA in plants, we investigated the effects of different host species, genes and promoters on EPA biosynthesis. Zero-erucic acid Brassica carinata appeared to be an outstanding host species for EPA production, with EPA levels in transgenic seed of this line reaching up to 25%. Two novel genes, an 18-carbon omega3 desaturase (CpDesX) from Claviceps purpurea and a 20-carbon omega3 desaturase (Pir-omega3) from Pythium irregulare, proved to be very effective in increasing EPA levels in high-erucic acid B. carinata. The conlinin1 promoter from flax functioned reasonably well in B. carinata, and can serve as an alternative to the napin promoter from B. napus. In summary, the judicious selection of host species and promoters, together with the inclusion of genes that enhance the basic very long chain polyunsaturated fatty acid biosynthetic pathway, can greatly influence the production of EPA in plants.
Authors:
Bifang Cheng; Guohai Wu; Patricia Vrinten; Kevin Falk; Joerg Bauer; Xiao Qiu
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2009-07-07
Journal Detail:
Title:  Transgenic research     Volume:  19     ISSN:  1573-9368     ISO Abbreviation:  Transgenic Res.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-02-16     Completed Date:  2010-05-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9209120     Medline TA:  Transgenic Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  221-9     Citation Subset:  IM    
Affiliation:
Bioriginal Food and Science Corporation, 110 Gymnasium Place, Saskatoon, SK S7N 0W9, Canada.
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MeSH Terms
Descriptor/Qualifier:
Biotechnology / methods*
Brassica / classification,  genetics*,  growth & development,  metabolism
Claviceps / enzymology,  genetics
Eicosapentaenoic Acid / biosynthesis*
Erucic Acids / metabolism
Fatty Acid Desaturases / genetics,  metabolism*
Gene Expression Regulation, Plant
Humans
Plants, Genetically Modified / enzymology,  genetics*,  metabolism*
Promoter Regions, Genetic
Pythium / enzymology,  genetics
Seeds / genetics,  metabolism
Species Specificity
Transformation, Genetic
Chemical
Reg. No./Substance:
0/Erucic Acids; 1072-39-5/erucic acid; 1553-41-9/Eicosapentaenoic Acid; EC 1.14.19.-/Fatty Acid Desaturases

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