| Towards the production of high levels of eicosapentaenoic acid in transgenic plants: the effects of different host species, genes and promoters. | |
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MedLine Citation:
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PMID: 19582587 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Eicosapentaenoic acid (EPA, 20:5n-3) plays an important role in many aspects of human health. In our efforts towards producing high levels of EPA in plants, we investigated the effects of different host species, genes and promoters on EPA biosynthesis. Zero-erucic acid Brassica carinata appeared to be an outstanding host species for EPA production, with EPA levels in transgenic seed of this line reaching up to 25%. Two novel genes, an 18-carbon omega3 desaturase (CpDesX) from Claviceps purpurea and a 20-carbon omega3 desaturase (Pir-omega3) from Pythium irregulare, proved to be very effective in increasing EPA levels in high-erucic acid B. carinata. The conlinin1 promoter from flax functioned reasonably well in B. carinata, and can serve as an alternative to the napin promoter from B. napus. In summary, the judicious selection of host species and promoters, together with the inclusion of genes that enhance the basic very long chain polyunsaturated fatty acid biosynthetic pathway, can greatly influence the production of EPA in plants. |
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Authors:
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Bifang Cheng; Guohai Wu; Patricia Vrinten; Kevin Falk; Joerg Bauer; Xiao Qiu |
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Publication Detail:
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Type: Evaluation Studies; Journal Article Date: 2009-07-07 |
Journal Detail:
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Title: Transgenic research Volume: 19 ISSN: 1573-9368 ISO Abbreviation: Transgenic Res. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-02-16 Completed Date: 2010-05-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9209120 Medline TA: Transgenic Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 221-9 Citation Subset: IM |
Affiliation:
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Bioriginal Food and Science Corporation, 110 Gymnasium Place, Saskatoon, SK S7N 0W9, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biotechnology
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methods* Brassica / classification, genetics*, growth & development, metabolism Claviceps / enzymology, genetics Eicosapentaenoic Acid / biosynthesis* Erucic Acids / metabolism Fatty Acid Desaturases / genetics, metabolism* Gene Expression Regulation, Plant Humans Plants, Genetically Modified / enzymology, genetics*, metabolism* Promoter Regions, Genetic Pythium / enzymology, genetics Seeds / genetics, metabolism Species Specificity Transformation, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Erucic Acids; 1072-39-5/erucic acid; 1553-41-9/Eicosapentaenoic Acid; EC 1.14.19.-/Fatty Acid Desaturases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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