Document Detail


Towards pancreatic cancer diagnosis using EIS biochips.
MedLine Citation:
PMID:  23287869     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive and lethal cancers in Europe and the United States. It has a very low 5 years-survival rate and its diagnosis is often late and imprecise due to the lack of specificity of currently used markers for PDAC. As previously demonstrated PDAC patients' sera may contain autoantibodies towards phosphorylated α-enolase (ENOA), which in combination with other standard markers can increase specificity in diagnosis of PDAC. In this context we realized a microfluidic platform with integrated EIS biosensors. We achieved a specific antibodies detection by immobilizing onto electrodes peptides corresponding to a portion of ENOA. Phosphorylation of peptides was found to influence the recognition of antibodies in PDAC patients' sera detected by the developed biochip thus validating the EIS technique as a strong tool for quick, cost-saving and label-free analysis of serum samples. Biochip results are in agreement with those from traditional techniques, such as ELISA and western blot, but measurements are much more sensitive and specific, increasing the possibility of PDAC diagnosis. In addition this approach is faster and more reproducible compared to traditional techniques making the developed biochips ideal for a quick, cost-saving and label-free analysis of serum samples.
Authors:
Maria Serena Chiriacò; Elisabetta Primiceri; Anna Grazia Monteduro; Anna Bove; Stefano Leporatti; Michela Capello; Sammy Ferri-Borgogno; Ross Rinaldi; Francesco Novelli; Giuseppe Maruccio
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-4
Journal Detail:
Title:  Lab on a chip     Volume:  -     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
NNL Istituto Nanoscienze - CNR and Dipartimento di Matematica e Fisica "Ennio De Giorgi", Scuola Superiore ISUFI, Università del Salento, Via per Arnesano, I-73100 Lecce, Italy. serena.chiriaco@unisalento.it giuseppe.maruccio@unisalento.it.
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