Document Detail


Towards a metabolic engineering strain "commons": An Escherichia coli platform strain for ethanol production.
MedLine Citation:
PMID:  23322646     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In the genome-engineering era it is increasingly important that researchers have access to a common set of platform strains that can serve as debugged production chassis and the basis for applying new metabolic engineering strategies for modeling and characterizing flux, engineering complex traits, and optimizing overall performance. Here we describe such a platform strain of E. coli engineered for ethanol production. Starting with a fully characterized host strain (BW25113), we site-specifically integrated the genes required for homoethanol production under the control of a strong inducible promoter into the genome and deleted the genes encoding four enzymes from competing pathways. This strain is capable of producing >30 g/L of ethanol in minimal media with < 2 g/L produced of any fermentative byproduct. Using this platform strain, we tested previously identified ethanol tolerance genes and found that while tolerance was improved under certain conditions, any effect on ethanol production or tolerance was lost when grown under production conditions. Thus, our findings reinforce the need for a metabolic engineering "commons" that could provide a set of platform strains for use in more sophisticated genome-engineering strategies. Towards this end, we have made this production strain available to the scientific community. Biotechnol. Bioeng. © 2013 Wiley Periodicals, Inc.
Authors:
Lauren B A Woodruff; Brian L May; Joseph R Warner; Ryan T Gill
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-15
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  -     ISSN:  1097-0290     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
Affiliation:
Department of Chemical and Biological Engineering, University of Colorado at Boulder, Jennie Smoly Caruthers Biotechnology Building, UCB 596, Boulder, CO 80309, USA.
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