Document Detail


Towards the mechanism of altered nutrients uptake in renal brush border membrane vesicles from pyelonephritic rats.
MedLine Citation:
PMID:  3454649     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Affinity constant (Km) of D-glucose, L-alanine, L-aspartate, L-lysine, L-proline and nutrients coupled Na+ were determined in renal brush border membrane vesicles prepared from control and pyelonephritic rats. The Km of D-glucose, amino acids and nutrients coupled Na+ was noted to be significantly increased (p less than 0.001) in experimental animals. The Vmax of D-glucose and amino acids was determined at different concentrations of nutrients keeping extravesicular Na+ constant or at different concentrations of extravesicular Na+ keeping nutrient concentration constant. In the experimental rats the Vmax decreased significantly (p less than 0.01) when compared to control. The increased Km and decreased Vmax may be one of the underlying mechanism leading to decrease in the uptake of D-glucose and amino acids.
Authors:
U C Garg; N K Ganguly; R Bhatnagar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry international     Volume:  14     ISSN:  0158-5231     ISO Abbreviation:  Biochem. Int.     Publication Date:  1987 May 
Date Detail:
Created Date:  1988-09-15     Completed Date:  1988-09-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100311     Medline TA:  Biochem Int     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  949-55     Citation Subset:  IM    
Affiliation:
Department of Experimental Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / pharmacokinetics*
Animals
Female
Glucose / pharmacokinetics*
Kidney / metabolism*
Microvilli / metabolism
Pyelonephritis / metabolism*
Rats
Rats, Inbred Strains
Sodium / pharmacokinetics
Chemical
Reg. No./Substance:
0/Amino Acids; 50-99-7/Glucose; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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