| Towards identifying the full set of genes expressed during cassava post-harvest physiological deterioration. | |
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MedLine Citation:
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PMID: 17318318 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Storage roots of cassava (Manihot esculenta Crantz) exhibit a rapid post-harvest physiological deterioration (PPD) response that can occur within 24-72 h of harvest. PPD is an enzymatically mediated oxidative process with parallels to plant wound, senescence and defence responses. To characterise those genes that show significant change in expression during the PPD response we have used cDNA microarray technology to carry out a large-scale analysis of the cassava root transcriptome during the post-harvest period. We identified 72 non-redundant expressed sequence tags which showed altered regulation during the post-harvest period. Of these 63 were induced, whilst 9 were down-regulated. RNA blot analysis of selected genes was used to verify and extend the microarray data. Additional microarray hybridisation experiments allowed the identification of 21 root-specific and 24 root-wounding-specific sequences. Many of the up-regulated and PPD-specific expressed sequence tags were predicted to play a role in cellular processes including reactive oxygen species turnover, cell wall repair, programmed cell death, ion, water or metabolite transport, signal transduction or perception, stress response, metabolism and biosynthesis, and activation of protein synthesis. |
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Authors:
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Kim Reilly; Diana Bernal; Diego F Cortés; Rocío Gómez-Vásquez; Joe Tohme; John R Beeching |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-02-22 |
Journal Detail:
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Title: Plant molecular biology Volume: 64 ISSN: 0167-4412 ISO Abbreviation: Plant Mol. Biol. Publication Date: 2007 May |
Date Detail:
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Created Date: 2007-04-11 Completed Date: 2007-06-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9106343 Medline TA: Plant Mol Biol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 187-203 Citation Subset: IM |
Affiliation:
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Department of Biology and Biochemistry, University of Bath, Bath, BA2 7AY, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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genetics Biological Transport / genetics Cell Wall / genetics, metabolism Expressed Sequence Tags Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant* Manihot / genetics*, growth & development Oligonucleotide Array Sequence Analysis Plant Proteins / genetics, metabolism Plant Roots / genetics, growth & development Reactive Oxygen Species / metabolism Signal Transduction / genetics Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Plant Proteins; 0/Reactive Oxygen Species |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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