| Towards detection and identification of circulating tumour cells using Raman spectroscopy. | |
| | |
MedLine Citation:
|
PMID: 20941448 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Body fluids are easily accessible and contain valuable indices for medical diagnosis. Fascinating tools are tumour cells circulating in the peripheral blood of cancer patients. As these cells are extremely rare, they constitute a challenge for clinical diagnostics. In this contribution we present the Raman spectroscopic-based identification of different single cells in suspension that are found in peripheral blood of cancer patients including healthy cells like leukocytes and erythrocytes, and tumour cells like leukaemic cells and cells originating from solid tumours. Leukocytes and erythrocytes were isolated from the peripheral blood of healthy donors while myeloid leukaemia cells (OCI-AML3) and breast carcinoma derived cells (MCF-7, BT-20) were obtained from cell cultures. A laser emitting 785 nm light was used for optical trapping the single cells in the laser focus and to excite the Raman spectrum. Support vector machines were applied to develop a supervised classification model with spectra of 1210 cells originating from three different donors and three independent cultivation batches. Distinguishing tumour cells from healthy cells was achieved with a sensitivity of >99.7% and a specificity of >99.5%. In addition, the correct cell types were predicted with an accuracy of approximately 92%. |
| | |
Authors:
|
U Neugebauer; T Bocklitz; J H Clement; C Krafft; J Popp |
Related Documents
:
|
7962608 - Pcr in situ hybridisation detection of hpv 16 in fixed caski and fixed siha cell lines. 18374478 - Lysyl oxidase (lox) mrna expression and genes of the differentiated osteoblastic phenot... 3500878 - Patterns of in vitro behaviour characterizing cells of spontaneously metastasizing k2m ... 11720448 - Drug-induced alterations in tumour perfusion yield increases in tumour cell radiosensit... 7829228 - Down-regulation of collagen xii in transformed mesenchymal cells. 21183028 - Selenomethionine increases proliferation and reduces apoptosis in bovine mammary epithe... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-13 |
Journal Detail:
|
Title: The Analyst Volume: 135 ISSN: 1364-5528 ISO Abbreviation: Analyst Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-11-18 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372652 Medline TA: Analyst Country: England |
Other Details:
|
Languages: eng Pagination: 3178-82 Citation Subset: IM |
Affiliation:
|
Institute of Photonic Technology, Albert-Einstein-Str. 9, D-07745 Jena, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Anti-influenza agents from Traditional Chinese Medicine.
Next Document: Push-pull nitrile ligands exhibit specific hydration patterns.