Document Detail


Towards covering immunological genes with highly informative markers: a trans-species approach.
MedLine Citation:
PMID:  8400718     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To establish a highly informative screening system for immunologically relevant genes ("immunoprinting") we co-amplified via polymerase chain reaction (PCR) polymorphic exons plus adjacent intronic simple repetitive dinucleotide stretches in the T-cell receptor (Tcr) Vb6 and Major Histocompatibility Complex (MHC)-DRB loci in man and several ungulate species. In both gene families the basic structure of the simple repeat was found to be preserved for more than 70 x 10(6) years in all investigated species. The simple repeats exhibit extensive length variability. Distinct exon sequences are correlated with a defined repeat length and substructure. In addition, PCR and the oligonucleotides for typing were applicable to a broad range of species from different mammalian orders. Multiplex PCR of different members of the Tcr Vb6 family and MHC-DRB resulted in a complex pattern similar to an oligolocus fingerprint. Hence immunoprinting can be employed for searching for associations of immunologically relevant genes with diseases even across species barriers.
Authors:
J Buitkamp; W Schwaiger; C Epplen; M Gomolka; E Weyers; J T Epplen
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  EXS     Volume:  67     ISSN:  1023-294X     ISO Abbreviation:  EXS     Publication Date:  1993  
Date Detail:
Created Date:  1993-11-05     Completed Date:  1993-11-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9204529     Medline TA:  EXS     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  87-102     Citation Subset:  IM    
Affiliation:
Ruhr-University, Bochum, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cattle
DNA / genetics
DNA Fingerprinting
Female
Genes, MHC Class II*
Genetic Markers
Goats
HLA-DR6 Antigen / genetics
Humans
Male
Molecular Sequence Data
Pedigree
Polymerase Chain Reaction
Receptors, Antigen, T-Cell, alpha-beta / genetics
Repetitive Sequences, Nucleic Acid
Sheep
Species Specificity
Chemical
Reg. No./Substance:
0/Genetic Markers; 0/HLA-DR6 Antigen; 0/Receptors, Antigen, T-Cell, alpha-beta; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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