| Towards covering immunological genes with highly informative markers: a trans-species approach. | |
| | |
MedLine Citation:
|
PMID: 8400718 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
To establish a highly informative screening system for immunologically relevant genes ("immunoprinting") we co-amplified via polymerase chain reaction (PCR) polymorphic exons plus adjacent intronic simple repetitive dinucleotide stretches in the T-cell receptor (Tcr) Vb6 and Major Histocompatibility Complex (MHC)-DRB loci in man and several ungulate species. In both gene families the basic structure of the simple repeat was found to be preserved for more than 70 x 10(6) years in all investigated species. The simple repeats exhibit extensive length variability. Distinct exon sequences are correlated with a defined repeat length and substructure. In addition, PCR and the oligonucleotides for typing were applicable to a broad range of species from different mammalian orders. Multiplex PCR of different members of the Tcr Vb6 family and MHC-DRB resulted in a complex pattern similar to an oligolocus fingerprint. Hence immunoprinting can be employed for searching for associations of immunologically relevant genes with diseases even across species barriers. |
| | |
Authors:
|
J Buitkamp; W Schwaiger; C Epplen; M Gomolka; E Weyers; J T Epplen |
Related Documents
:
|
1974878 - Informativeness of human (dc-da)n.(dg-dt)n polymorphisms. 1346778 - Mouse genomic dna sequences homologous to sea urchin tu elements are genetically stable... 15777738 - The first characterisation of the overall variability of repetitive units in a species ... 3039298 - Analysis of the f plasmid centromere. 19096508 - Frequent toggling between alternative amino acids is driven by selection in hiv-1. 17715288 - Novel mutation in splicing donor of dystrophin gene first exon in a patient with dilate... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: EXS Volume: 67 ISSN: 1023-294X ISO Abbreviation: EXS Publication Date: 1993 |
Date Detail:
|
Created Date: 1993-11-05 Completed Date: 1993-11-05 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9204529 Medline TA: EXS Country: SWITZERLAND |
Other Details:
|
Languages: eng Pagination: 87-102 Citation Subset: IM |
Affiliation:
|
Ruhr-University, Bochum, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Base Sequence Cattle DNA / genetics DNA Fingerprinting Female Genes, MHC Class II* Genetic Markers Goats HLA-DR6 Antigen / genetics Humans Male Molecular Sequence Data Pedigree Polymerase Chain Reaction Receptors, Antigen, T-Cell, alpha-beta / genetics Repetitive Sequences, Nucleic Acid Sheep Species Specificity |
| Chemical | |
Reg. No./Substance:
|
0/Genetic Markers; 0/HLA-DR6 Antigen; 0/Receptors, Antigen, T-Cell, alpha-beta; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Iterons of stringently controlled plasmids and DNA fingerprinting.
Next Document: Autism and related behaviours.