| Toward an international standard for PCR-based detection of food-borne thermotolerant Campylobacters: validation in a multicenter collaborative trial. | |
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MedLine Citation:
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PMID: 12957959 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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As part of a European research project, the performance of a PCR assay to detect food-borne thermotolerant campylobacters (Campylobacter jejuni, C. coli, and C. lari) was evaluated through an international collaborative trial involving 12 participating laboratories. DNA from 10 target and 8 nontarget strains was tested, and the results were reported as the presence of a positive signal after gel electrophoresis. The overall inclusivity (sensitivity) was 93.7%, and the exclusivity (specificity) was 100%. The results indicate that the assay can become an international standard and can be confidently applied in microbiological laboratories. |
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Authors:
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P S Lübeck; N Cook; M Wagner; P Fach; J Hoorfar |
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Publication Detail:
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Type: Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 69 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2003 Sep |
Date Detail:
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Created Date: 2003-09-05 Completed Date: 2004-01-12 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 5670-2 Citation Subset: IM |
Affiliation:
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Danish Veterinary Institute, DK-1790 Copenhagen V, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Arcobacter
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isolation & purification Campylobacter / classification, isolation & purification* Campylobacter coli / classification, isolation & purification Campylobacter fetus / classification, isolation & purification Campylobacter jejuni / classification, isolation & purification Campylobacter lari / classification, isolation & purification Food Microbiology* / standards Polymerase Chain Reaction / methods, standards* Quality Control Reproducibility of Results Salmonella enterica / isolation & purification |
| Comments/Corrections | |
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