Document Detail


Toward a definition of "fresh" whole blood: an in vitro characterization of coagulation properties in refrigerated whole blood for transfusion.
MedLine Citation:
PMID:  20663116     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The hemostatic property of "fresh" whole blood (WB) has been observed in military application and cardiac surgery and is associated with reduced blood loss, transfusion requirements, and donor exposures. The time from donation to transfusion defining "fresh" has not been systematically studied. We undertook an in vitro study of coagulation properties of refrigerated WB stored for 31 days.
STUDY DESIGN AND METHODS: Twenty-one WB units were obtained from healthy volunteer donors and stored under standard AABB refrigerated conditions. Samples were obtained on the day after donation and again on Days 2, 4, 7, 11, 14, 17, 21, 24, and 31. Tests included complete blood count, pH, pO2, pCO2, glucose, lactate, thromboelastography (TEG), and platelet function by light transmission aggregometry (LTA).
RESULTS: There was progressive decline in pH, pO2, glucose, and sodium, but progressive increase in potassium, pCO2, and lactate. TEG variables in all units were normal through Day 11; abnormal values in some variables in some units began on Day 14. Final aggregation levels exhibited no change from Day 1 to Day 21 with adenosine diphosphate and epinephrine, but a decline with collagen (Day 7) and ristocetin (Day 17).
CONCLUSION: This in vitro study of coagulation properties demonstrates preservation of normal integrated coagulation function to a minimum of 11 days under standard conditions of refrigerated storage of WB for transfusion. These observations strongly suggest that the hemostatic quality of WB may extend beyond current transfusion practices. If confirmed clinically, this would increase availability and extend benefits of reduced donor exposure and transfusion requirements.
Authors:
David Jobes; Yanika Wolfe; Daniel O'Neill; Jennifer Calder; Lisa Jones; Deborah Sesok-Pizzini; X Long Zheng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transfusion     Volume:  51     ISSN:  1537-2995     ISO Abbreviation:  Transfusion     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-11     Completed Date:  2011-02-08     Revised Date:  2013-12-19    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  43-51     Citation Subset:  IM    
Copyright Information:
© 2010 American Association of Blood Banks.
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MeSH Terms
Descriptor/Qualifier:
Blood Cell Count
Blood Coagulation
Blood Glucose
Blood Transfusion / methods*
Carbon Dioxide / blood
Humans
Hydrogen-Ion Concentration
Lactic Acid / blood
Oxygen / blood
Partial Pressure
Platelet Function Tests
Refrigeration / methods*
Thrombelastography
Grant Support
ID/Acronym/Agency:
P01 HL074124/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Blood Glucose; 142M471B3J/Carbon Dioxide; 33X04XA5AT/Lactic Acid; S88TT14065/Oxygen
Comments/Corrections
Comment In:
Transfusion. 2011 Jan;51(1):5-7   [PMID:  21219320 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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