Document Detail

Totipotency, pluripotency and nuclear reprogramming.
MedLine Citation:
PMID:  19343304     Owner:  NLM     Status:  MEDLINE    
Mammalian development commences with the totipotent zygote which is capable of developing into all the specialized cells that make up the adult animal. As development unfolds, cells of the early embryo proliferate and differentiate into the first two lineages, the pluripotent inner cell mass and the trophectoderm. Pluripotent cells can be isolated, adapted and propagated indefinitely in vitro in an undifferentiated state as embryonic stem cells (ESCs). ESCs retain their ability to differentiate into cells representing the three major germ layers: endoderm, mesoderm or ectoderm or any of the 200+ cell types present in the adult body. Since many human diseases result from defects in a single cell type, pluripotent human ESCs represent an unlimited source of any cell or tissue type for replacement therapy thus providing a possible cure for many devastating conditions. Pluripotent cells resembling ESCs can also be derived experimentally by the nuclear reprogramming of somatic cells. Reprogrammed somatic cells may have an even more important role in cell replacement therapies since the patient's own somatic cells can be used for reprogramming thereby eliminating immune based rejection of transplanted cells. In this review, we summarize two major approaches to reprogramming: (1) somatic cell nuclear transfer and (2) direct reprogramming using genetic manipulations.
Shoukhrat Mitalipov; Don Wolf
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Advances in biochemical engineering/biotechnology     Volume:  114     ISSN:  0724-6145     ISO Abbreviation:  Adv. Biochem. Eng. Biotechnol.     Publication Date:  2009  
Date Detail:
Created Date:  2009-09-25     Completed Date:  2014-05-05     Revised Date:  2014-09-20    
Medline Journal Info:
Nlm Unique ID:  8307733     Medline TA:  Adv Biochem Eng Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  185-99     Citation Subset:  IM    
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MeSH Terms
Cell Culture Techniques
Cell Differentiation
Cell- and Tissue-Based Therapy / methods
Embryonic Stem Cells / cytology,  metabolism
Epigenesis, Genetic
Nuclear Reprogramming / genetics*
Nuclear Transfer Techniques*
Pluripotent Stem Cells / cytology*,  metabolism
Signal Transduction
Totipotent Stem Cells / cytology*,  metabolism
Grant Support
P51 RR000163/RR/NCRR NIH HHS; P51 RR000163-496074/RR/NCRR NIH HHS; P51 RR000163-496133/RR/NCRR NIH HHS; P51 RR000163-496134/RR/NCRR NIH HHS; P51 RR000163-496136/RR/NCRR NIH HHS; P51 RR000163-496137/RR/NCRR NIH HHS; P51 RR000163-496186/RR/NCRR NIH HHS; P51 RR000168/RR/NCRR NIH HHS; P51 RR000168-466897/RR/NCRR NIH HHS; R01 HD057121/HD/NICHD NIH HHS; R01 HD057121-01A2/HD/NICHD NIH HHS; R01 NS044330/NS/NINDS NIH HHS; R01 NS044330-05/NS/NINDS NIH HHS

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