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Total fatty acid content of the plasma membrane of Saccharomyces cerevisiae is more responsible for ethanol tolerance than the degree of unsaturation.
MedLine Citation:
PMID:  21063748     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The effect of change in unsaturated fatty acid composition on ethanol tolerance in Saccharomyces cerevisiae overexpressing ScOLE1 (∆9 fatty acid desaturase gene of S. cerevisiae), CaFAD2 (∆12 fatty acid desaturase gene of Candida albicans), or CaFAD3 (ω3 fatty acid desaturase gene of C. albicans) was examined. ScOLE1 over-expression increased the total unsaturated fatty acid content and enhanced ethanol tolerance, compared with a control strain. In contrast, overexpression of CaFAD2 and CaFAD3, which led to production of linoleic acid (18:2) and α-linolenic acid (18:3), respectively, neither changed total unsaturated fatty acids nor enhanced ethanol tolerance. The total unsaturated fatty acid content rather than the degree of unsaturation is thus an important factor for ethanol tolerance.
Authors:
Hyun-Soo Kim; Na-Rae Kim; Wonja Choi
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Publication Detail:
Type:  Journal Article     Date:  2010-11-10
Journal Detail:
Title:  Biotechnology letters     Volume:  33     ISSN:  1573-6776     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  509-15     Citation Subset:  IM    
Affiliation:
Department of Life Science, College of Natural Sciences, Ewha Womans University, 11-1, Daehyun-dong, Seodaemun-gu, Seoul, 120-750, South Korea.
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