| Topography of the membrane-binding domain of cytochrome b5 in lipids by Fourier-transform infrared spectroscopy. | |
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MedLine Citation:
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PMID: 2176852 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fourier-transform infrared spectroscopy was used to examine the secondary structure of the membrane-binding domain (nonpolar peptide) of rabbit liver cytochrome b5 in D2O and in the presence of phospholipids and deoxycholate. In all situations, the predominant structure was alpha helix, but an examination of the components of the amide I band in the spectrum of the nonpolar peptide showed that the major peak was shifted from 1655 cm-1 in the lipids to 1650 cm-1 in deoxycholate. This shift to lower frequency, together with a decrease in intensity of the amide II band, is indicative of N-H to N-D exchange of the peptide backbone. A semiquantitative analysis indicated that the alpha helix of the peptide is over 95% exchanged in the presence of deoxycholate but is only 10% exchanged in the presence of lipid. These data suggest that the membrane-inserted portion of the peptide is alpha helical and is largely protected from N-H to N-D exchange by the bilayer. We suggest that this technique appears to provide a general method for determining the type of secondary structure involved in membrane interaction and the percentage of this structure which is involved in the interaction. |
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Authors:
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P W Holloway; C Buchheit |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemistry Volume: 29 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1990 Oct |
Date Detail:
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Created Date: 1991-02-25 Completed Date: 1991-02-25 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 9631-7 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of Virginia School of Medicine, Charlottesville 22908. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Binding Sites Circular Dichroism Cytochromes b5 / chemistry, metabolism* Deoxycholic Acid / pharmacology* Deuterium Deuterium Oxide Fourier Analysis Liver / metabolism Membranes / metabolism Molecular Sequence Data Phospholipids / pharmacology* Protein Conformation Rabbits Spectrometry, Fluorescence Spectrophotometry, Infrared / methods Water |
| Grant Support | |
ID/Acronym/Agency:
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GM 23858/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Phospholipids; 7732-18-5/Water; 7782-39-0/Deuterium; 7789-20-0/Deuterium Oxide; 83-44-3/Deoxycholic Acid; 9035-39-6/Cytochromes b5 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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