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Topographical localization of the receptors for luteinizing hormone-releasing hormone on the surface of dissociated pituitary cells.
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MedLine Citation:
PMID:  233747     Owner:  NLM     Status:  MEDLINE    
A derivative of the hypothalamic peptide luteinizing hormone-releasing hormone (LHRH) has been coupled to ferritin and the conjugate purified by gel chromatography. In its ability to stimulate the secretion of luteinizing hormone from pituitary cells in vitro, the conjugate has the same potency and specificity as the native peptide. When dissociated pituitary cells maintained in short-term culture are lightly fixed with formaldehyde and then incubated with the conjugate, examination in the electron microscope shows an even distribution of ferritin particles over the free cell surface of the gonadotrophin cells. This binding appears to be specific for the LHRH receptor since it is prevented by a 10-fold excess of native peptide. In addition to the gonadotrophin cells, some somatotrophin and thyrotrophin cells bind conjugate on their free surfaces under similar conditions. If living cells are incubated with the conjugate for 15 min, the bound conjugate becomes aggregated and then concentrated in one localized area of the cell surface. In this area, which lies immediately above the juxtanuclear Golgi complex, the plasma membrane is frequently invaginated in a manner which suggests that the bound, aggregated conjugate is internalized by endocytosis.
C R Hopkins; H Gregory
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  75     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1977 Nov 
Date Detail:
Created Date:  1987-04-21     Completed Date:  1987-04-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  528-40     Citation Subset:  IM    
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MeSH Terms
Cell Membrane / metabolism,  ultrastructure
Gonadotropin-Releasing Hormone / metabolism*
Pituitary Gland / metabolism*,  ultrastructure
Rats, Inbred Strains
Receptors, LHRH / metabolism*
Reg. No./Substance:
0/Receptors, LHRH; 33515-09-2/Gonadotropin-Releasing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 11 Year: 1977
Volume: 75 Issue: 2
First Page: 528 Last Page: 540
ID: 2109944
Publisher Id: 87137941
PubMed Id: 233747

Topographical localization of the receptors for luteinizing hormone- releasing hormone on the surface of dissociated pituitary cells

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