| Topographical distribution of a membrane-inserted fluorescent phospholipid analogue during cell fusion. | |
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MedLine Citation:
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PMID: 6301868 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Potential alterations in the transbilayer distribution of lipid molecules during cell-cell fusion were studied, using the fluorescent phospholipid analogue 1-acyl, 2-(N-4-nitrobenzo-2-oxa-1,3-diazole)-aminocaproyl phosphatidylcholine (C6-NBD-PC). The fluophore was inserted into the outer leaflet of the plasma membrane of Chinese hamster fibroblasts from an exogenous source and cell-cell fusion was induced either with Sendai virus or polyethylene glycol (PEG). After fusion, the cells were examined under a fluorescence microscope and the pool of tagged lipid molecules in the external monolayer was determined quantitatively. The results showed that in contrast to PEG-induced cell fusion, substantial redistribution of the lipid marker occurred when cell fusion was induced by Sendai virus and it was estimated that approx. 40% of exogenously supplied lipid was internalized. The possible mechanism causing lipid redistribution in the case of Sendai virus-induced cell fusion is discussed. |
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Authors:
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D Hoekstra |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Experimental cell research Volume: 144 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 1983 Apr |
Date Detail:
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Created Date: 1983-06-23 Completed Date: 1983-06-23 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 482-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Fusion* Cell Line Cricetinae Fibroblasts Fluorescent Dyes / metabolism Liposomes Membrane Lipids / metabolism* Parainfluenza Virus 1, Human / metabolism, physiology Phosphatidylcholines Polyethylene Glycols / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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GM 22942/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Liposomes; 0/Membrane Lipids; 0/Phosphatidylcholines; 0/Polyethylene Glycols; 10015-85-7/1,2-oleoylphosphatidylcholine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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