Document Detail

Topographical associations between islet endocrine cells and duct epithelial cells in the adult human pancreas.
MedLine Citation:
PMID:  18221396     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: The pancreatic ducts, endocrine islets and exocrine acini are three functionally related components. From birth to adulthood, the islets and ducts are regarded as independent entities. The objective of this study is to investigate the topographical associations between the islet endocrine cells and duct epithelial cells in the adult human pancreas. MATERIALS AND METHODS: Panels of immunomarkers for the exocrine acinar cells (amylase), duct cells [cytokeratin 19 (CK19)], endocrine cells (chromogranin A, neurone specific enolase, synaptophysin) and islet hormones (glucagon, insulin, somatostatin, pancreatic polypeptide) were applied to sequential pancreatic tissue sections obtained from autopsy specimens of 10-nondiabetic human adults. Double immunofluorescent staining with CK19 and islet hormones was performed to confirm the islet to duct interrelationship. RESULTS: Sequential sectioning and immunostaining showed that 45% of the 172 islets examined appeared as single endocrine cell units or small clusters of < 10 endocrine cells on at least one plane of section. A topographical association was found between the islet endocrine cells and duct epithelial cells. Topographical associations with CK 19-stained duct cells occurred in 10.9% of the islet insulin-containing beta-cells and in 8.9% of the islet glucagon-producing alpha-cells. The frequency of topographical associations increased toward the more distally located duct systems. The CK19-stained duct cells and amylase-labelled acinar cells were less frequently in association with other islet hormone-producing cells. CONCLUSIONS: Topographical associations between islet endocrine cells and pancreatic duct cells are frequent in adult human pancreas. The islet-duct association suggests possible functional interactions between the two interrelated pancreatic compartments.
Hai-Lu Zhao; Yi Sui; Jing Guan; Fernand M M Lai; Xue-Mei Gu; Lan He; Xun Zhu; Dewi K Rowlands; Gang Xu; Peter C Y Tong; Juliana C N Chan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-21
Journal Detail:
Title:  Clinical endocrinology     Volume:  69     ISSN:  1365-2265     ISO Abbreviation:  Clin. Endocrinol. (Oxf)     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-10-17     Completed Date:  2009-07-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0346653     Medline TA:  Clin Endocrinol (Oxf)     Country:  England    
Other Details:
Languages:  eng     Pagination:  400-6     Citation Subset:  IM    
Department of Medicine and Therapeutics, the Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong SAR, China.
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MeSH Terms
Aged, 80 and over
Biological Markers / metabolism
Endocrine Cells / cytology,  immunology,  metabolism
Epithelial Cells / cytology*,  immunology,  metabolism
Glucagon / metabolism
Insulin / metabolism
Islets of Langerhans / cytology*,  immunology,  metabolism
Middle Aged
Pancreas / cytology,  immunology,  metabolism
Pancreatic Ducts / cytology*,  immunology,  metabolism
Reg. No./Substance:
0/Biological Markers; 11061-68-0/Insulin; 9007-92-5/Glucagon

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