Document Detail

Topographic variations in rat brain oligodendrocyte morphology elucidated by injection of Lucifer Yellow in fixed tissue slices.
MedLine Citation:
PMID:  8852936     Owner:  NLM     Status:  MEDLINE    
Visualisation of oligodendrocytes by fluorochrome labelling in fresh tissue is a relatively recent innovation, but its widespread applicability in comparative analyses between different regions of the brain has been hampered by the limited survival time of excised preparations. We here applied the technique of impaling and injecting these cells with Lucifer Yellow in fixed tissue slices. Using confocal laser scanning microscopy, we reconstructed the three-dimensional forms of oligodendrocytes derived from the optic nerve, corpus callosum, cerebellum and spinal cord of young adult rats. Differences in shape and size of the cell body, in the number of internodal segments supplied by a single cell, as well in their spatial orientation, and in the thickness of the myelinated fibre, were observed between the four white matter tracts analysed.
E Weruaga-Prieto; P Eggli; M R Celio
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurocytology     Volume:  25     ISSN:  0300-4864     ISO Abbreviation:  J. Neurocytol.     Publication Date:  1996 Jan 
Date Detail:
Created Date:  1996-12-06     Completed Date:  1996-12-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0364620     Medline TA:  J Neurocytol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  19-31     Citation Subset:  IM    
Institute of Histology and General Embryology, University of Fribourg, Switzerland.
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MeSH Terms
Brain / cytology*
Cell Size
Fluorescent Dyes / diagnostic use
Isoquinolines / diagnostic use
Myelin Sheath
Oligodendroglia / cytology*,  ultrastructure
Rats, Inbred Strains
Tissue Fixation
Reg. No./Substance:
0/Fluorescent Dyes; 0/Isoquinolines; 77944-88-8/lucifer yellow

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