Document Detail


Topically applied nitric oxide induces T-lymphocyte infiltration in human skin, but minimal inflammation.
MedLine Citation:
PMID:  17914444     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nitric oxide (NO) plays an important role in the cutaneous response to UV radiation and in cutaneous inflammation. The presence of inducible NO synthase protein in a number of inflammatory dermatoses, coupled with the induction of an intense cutaneous inflammatory infiltrate following topical application of the NO donor-acidified nitrite (NO2(-)), has set the paradigm of NO being an inflammatory mediator in human skin. Using zeolite NO (Ze-NO), a chemically inert, pure NO donor, we have shown that NO per se produces little inflammation. Biologically, relevant doses of Ze-NO induce a dermal CD4-positive T-cell infiltrate and IFN-gamma secretion. In contrast acidified nitrite, releasing equal quantities of NO (measured by dermal microdialysis and cutaneous erythema), induces an intense epidermal infiltrate of macrophages with a similar dermal infiltrate of CD3-, CD4-, CD8-, and CD68-positive cells and neutrophils. Suction blisters were created in Ze-NO-treated and control skin. IFN-gamma, but not IL-4, was detected in Ze-NO-treated skin (mean control 0.1+/-0.07 pg mg(-1) protein, mean IFN-gamma 0.6+/-0.4 pg mg(-1) protein). We suggest that the potent inflammation induced by acidified NO2(-) is secondary to the release of additional mediators.
Authors:
Megan Mowbray; Xuejing Tan; Paul S Wheatley; Adriano G Rossi; Russell E Morris; Richard B Weller
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-04
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  128     ISSN:  1523-1747     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-15     Completed Date:  2008-02-19     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  352-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acids
Administration, Topical
Blister / chemically induced,  immunology,  pathology
Cell Movement / drug effects,  immunology
Dermatitis / immunology*,  pathology,  prevention & control*
Edema / chemically induced,  immunology,  pathology
Epidermis / drug effects,  immunology,  pathology
Erythema / chemically induced,  immunology,  pathology
Female
Humans
Inflammation Mediators / administration & dosage,  adverse effects
Langerhans Cells / drug effects,  pathology
Macrophages / drug effects,  pathology
Male
Neutrophils / drug effects,  pathology
Nitric Oxide / administration & dosage*,  adverse effects*,  immunology
Nitric Oxide Donors / administration & dosage,  adverse effects
Skin Ulcer / chemically induced,  immunology,  pathology
T-Lymphocytes / drug effects*,  pathology
Zeolites / administration & dosage,  adverse effects
Grant Support
ID/Acronym/Agency:
G0601481//Medical Research Council
Chemical
Reg. No./Substance:
0/Acids; 0/Inflammation Mediators; 0/Nitric Oxide Donors; 1318-02-1/Zeolites; 31C4KY9ESH/Nitric Oxide
Comments/Corrections
Comment In:
J Invest Dermatol. 2008 Feb;128(2):258-60   [PMID:  18195740 ]
Erratum In:
J Invest Dermatol. 2008 Oct;128(10):2546
Note: Rossi, Adriano G [added]

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