Document Detail


Topical ceramides neither enhance UVB-induced apoptosis in normal human keratinocytes nor affect viability in UVB-irradiated reconstructed human epidermis.
MedLine Citation:
PMID:  25078364     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Ceramides are the major lipid of lamellar sheets present in intercellular spaces of the stratum corneum contributing to epidermal barrier properties. Therefore ceramides and their analogues have been studied for barrier enhancing and water-holding properties for decades. In vitro studies have indicated cytotoxic potential for cell permeable ceramides thereby raising the question whether topical ceramide application might contribute to UVB-induced apoptosis. Phytosphingosine, N-hexanoyl-phytosphingosine and N-stearoylphytosphingosine (ceramide III) in concentrations ≤ 5μM have been used for co-stimulation with low (160J/m(2) ) or high (600J/m(2) ) UVB doses in sub-confluent basal and confluent differentiating keratinocytes. Significantly increased caspase-3 activity was observed in basal keratinocytes irradiated with 600J/m(2) UVB and in differentiating keratinocytes with both UVB doses. Co-stimulation with the named ceramides did not further increase (i) caspase-3 activity and (ii) nucleosomal fragmentation in differentiating keratinocytes. Moreover, co-stimulation with 1mM ceramides did not further affect viability/lactate dehydrogenase release in UVB-irradiated reconstructed human epidermis corroborating the safety of these ceramides. This article is protected by copyright. All rights reserved.
Authors:
S Grether-Beck; I Felsner; T Koehler; M Farwick; P Lersch; Av Rawlings; J Krutmann
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Publication Detail:
Type:  LETTER     Date:  2014-7-30
Journal Detail:
Title:  Experimental dermatology     Volume:  -     ISSN:  1600-0625     ISO Abbreviation:  Exp. Dermatol.     Publication Date:  2014 Jul 
Date Detail:
Created Date:  2014-7-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9301549     Medline TA:  Exp Dermatol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
This article is protected by copyright. All rights reserved.
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