Document Detail

Topical application of 5-aminolevulinic acid, DMSO and EDTA: protoporphyrin IX accumulation in skin and tumours of mice.
MedLine Citation:
PMID:  7623186     Owner:  NLM     Status:  MEDLINE    
Topical 5-aminolevulinic acid (ALA) application in three different creams was carried out on mice bearing subcutaneously transplanted C26 colon carcinoma. The creams contained (a) 20% ALA alone, (b) ALA with 2% dimethylsulphoxide (DMSO) and (c) ALA, DMSO and 2% edetic acid disodium salt (EDTA). Protoporphyrin IX (PP) production in the tumour and in the skin overlying the tumour was studied by two methods: laser-induced fluorescence (LIF) and chemical extraction. The kinetics of PP production in the skin and in the tumour, as studied by the LIF method, was similar for all three cream preparations. The PP fluorescence intensity in the tissues reached its maximum 4-6 h after application of the creams. Quantitative analysis showed that the PP concentration after treatment was more pronounced in the skin than in the tumour. The efficiency of porphyrin production in the skin by the creams used was in the following order: ALA-DMSO-EDTA > ALA-DMSO > ALA. In the tumour the enhancing effect of DMSO and EDTA on PP accumulation induced by ALA was observed mainly in the upper 2 mm section. However, the concentration of PP in the tumour was found to be approximately the same for ALA-DMSO and ALA-DMSO-EDTA cream combinations. The possible mechanisms of the effect of DMSO and EDTA are discussed.
Z Malik; G Kostenich; L Roitman; B Ehrenberg; A Orenstein
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of photochemistry and photobiology. B, Biology     Volume:  28     ISSN:  1011-1344     ISO Abbreviation:  J. Photochem. Photobiol. B, Biol.     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-08-31     Completed Date:  1995-08-31     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  8804966     Medline TA:  J Photochem Photobiol B     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  213-8     Citation Subset:  IM    
Life Sciences Department, Bar Ilan University, Ramat Gan, Israel.
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MeSH Terms
Administration, Topical
Aminolevulinic Acid / administration & dosage,  pharmacology*
Colonic Neoplasms / metabolism*
Dimethyl Sulfoxide / administration & dosage,  pharmacology*
Edetic Acid / administration & dosage,  pharmacology*
Mice, Inbred BALB C
Protoporphyrins / analysis,  metabolism*
Skin / drug effects,  metabolism*
Spectrometry, Fluorescence / methods
Time Factors
Reg. No./Substance:
0/Protoporphyrins; 106-60-5/Aminolevulinic Acid; 553-12-8/protoporphyrin IX; 60-00-4/Edetic Acid; 67-68-5/Dimethyl Sulfoxide

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