Document Detail


Topical anti-inflammatory effect of tirucallol, a triterpene isolated from Euphorbia lactea latex.
MedLine Citation:
PMID:  19577446     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Latex from Euphorbia lactea (Euphorbiaceae), a native Dominican medicinal plant, is claimed to be useful in the treatment of inflammation. Topical application of tirucallol, a tetracyclic triterpene isolated from Euphorbia lacteal latex, suppressed ear edema in the mouse model in a dose-dependent manner, as well as affecting the influx of polymorphonuclear cells in response to topical application of 12-O-tetradecanoylphorbol-acetate (TPA) in the mouse ear. In addition, the effect of tirucallol, on some macrophage functions was analyzed in vitro. Non-toxic concentrations of tirucallol potently inhibited nitrite production in lipopolysaccharide-stimulated macrophages. Western blot analysis showed that nitric oxide reduction was a consequence of the inhibition of inducible nitric oxide synthetase expression although tirucallol slightly affected to prostaglandin E(2) (PGE(2)) generation. The results of the study revealed that tirucallol (0.3%), present in Euphorbia lactea latex, exerts a topical anti-inflammatory effect in vivo, via a mechanism of action related to the neutrophil migration. On the other hand, it can be deduced that the mechanism of the anti-inflammatory activity of this triterpene is related to the control of the production of NO and its effect on the expression of iNOS.
Authors:
A Fernandez-Arche; M T Saenz; M Arroyo; R de la Puerta; M D Garcia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-03
Journal Detail:
Title:  Phytomedicine : international journal of phytotherapy and phytopharmacology     Volume:  17     ISSN:  1618-095X     ISO Abbreviation:  Phytomedicine     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-03     Completed Date:  2010-06-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9438794     Medline TA:  Phytomedicine     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  146-8     Citation Subset:  IM    
Copyright Information:
Published by Elsevier GmbH.
Affiliation:
Department of Pharmacology, Faculty of Pharmacy, University of Sevilla, Spain. yangzaichang@126.com
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MeSH Terms
Descriptor/Qualifier:
Administration, Topical
Animals
Anti-Inflammatory Agents / isolation & purification,  pharmacology*,  therapeutic use
Dinoprostone / biosynthesis
Disease Models, Animal
Dose-Response Relationship, Drug
Edema / drug therapy*,  metabolism
Euphorbia / chemistry*
Granulocytes / drug effects
Inflammation Mediators / metabolism*
Lanosterol / analogs & derivatives*,  isolation & purification,  pharmacology,  therapeutic use
Latex
Lipopolysaccharides
Macrophages / drug effects
Male
Mice
Neutrophil Infiltration / drug effects*
Nitric Oxide / metabolism
Nitric Oxide Synthase Type II / antagonists & inhibitors
Nitrites / antagonists & inhibitors
Phytotherapy*
Plant Extracts / chemistry,  pharmacology*,  therapeutic use
Tetradecanoylphorbol Acetate
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Inflammation Mediators; 0/Latex; 0/Lipopolysaccharides; 0/Nitrites; 0/Plant Extracts; 0/lanostan-8,24-dien-3-ol; 10102-43-9/Nitric Oxide; 16561-29-8/Tetradecanoylphorbol Acetate; 363-24-6/Dinoprostone; 79-63-0/Lanosterol; EC 1.14.13.39/Nitric Oxide Synthase Type II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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