| Topical application of gamma-tocopherol derivative prevents UV-induced skin pigmentation. | |
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MedLine Citation:
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PMID: 16755012 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We previously reported that a novel hydrophilic gamma-tocopherol (gamma-Toc) derivative, gamma-tocopheryl-N,N-dimethylglycinate hydrochloride (gamma-TDMG) gets converted to the antioxidant gamma-Toc in skin. We also found that this derivative displayed greater bioavailability than gamma-Toc itself. In the present study, we determined whether gamma-TDMG could reduce UV-induced skin pigmentation in brownish guinea pigs. gamma-TDMG (0.1 or 0.5%) was topically applied to the skin before and after it was exposed to UVB plus UVA (3 times/week for 1 week), and then 10 times/week for 4 weeks thereafter. Treatment with 0.5% gamma-TDMG resulted in significant skin lightening (70% of the pigmentation of irradiated controls). We also found that melanin synthesis was dose-dependently inhibited by gamma-TDMG in murine B16 melanoma cells. When gamma-TDMG or kojic acid (250 microM) were added to homogenates of B16 melanoma cells, their tyrosinase activity was significantly inhibited by approximately 40% and 75%, respectively. Mushroom tyrosinase activity was significantly inhibited by 200 microM gamma-Toc and kojic acid, but not gamma-TDMG. When B16 cells were incubated with 250 microM gamma-TDMG for 24 or 48 h, their intracellular gamma-Toc concentrations rose over 100 fold to 10.5 and 11.2 nmol/10(6) cells, respectively, suggesting that gamma-TDMG was rapidly converted to gamma-Toc in these cells and that their reduced melanin synthesis may have been due to the activity of gamma-Toc. Our data further suggest that the topical application of gamma-TDMG may be efficacious in preventing photo-induced skin pigmentation in humans. |
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Authors:
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Yuka Kuwabara; Tatsuya Watanabe; Shingo Yasuoka; Kohsuke Fukui; Jiro Takata; Yoshiharu Karube; Yuko Okamoto; Shin Asano; Eiko Katoh; Toshi Tsuzuki; Shizuko Kobayashi |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biological & pharmaceutical bulletin Volume: 29 ISSN: 1347-5215 ISO Abbreviation: Biol. Pharm. Bull. Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-06-06 Completed Date: 2010-02-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9311984 Medline TA: Biol Pharm Bull Country: Japan |
Other Details:
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Languages: eng Pagination: 1175-9 Citation Subset: IM |
Affiliation:
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Kyoritsu University of Pharmacy, Tokyo, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Topical Animals Cell Line, Tumor Glycine / administration & dosage, analogs & derivatives*, therapeutic use Guinea Pigs Melanins / biosynthesis* Skin / drug effects*, metabolism, radiation effects Skin Pigmentation / drug effects*, radiation effects Ultraviolet Rays* gamma-Tocopherol / administration & dosage, analogs & derivatives*, therapeutic use |
| Chemical | |
Reg. No./Substance:
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0/Melanins; 0/gamma-Tocopherol; 0/gamma-tocopherol-N,N-dimethylglycinate hydrochloride; 56-40-6/Glycine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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