Document Detail

Tonsillar biopsy and PrPSc detection in the preclinical diagnosis of scrapie.
MedLine Citation:
PMID:  9634704     Owner:  NLM     Status:  MEDLINE    
Preliminary findings have indicated that in naturally infected sheep, fully susceptible to scrapie (VRQ-homozygous), PrPSc can be detected in the tonsils approximately one year before the expected onset of clinical disease, whereas no immunostaining can be detected in animals with a semi-resistant genotype. This paper describes the technique for taking tonsillar biopsies from sheep and gives the results of the completed experiment. In another experiment PrPSc was detected even earlier in comparable VRQ-homozygous sheep born and raised in different surroundings. At three-and-a-half months of age no PrPSc could be detected in three homozygous susceptible sheep (VRQ/VRQ), but PrPSc was detected at four months in one similar sheep. At eight months of age all seven sampled VRQ/VRQ sheep showed positive immunostaining in the biopsies, but none of the biopsies from three VRQ/ARQ heterozygotes showed any immunostaining; they were positive when sampled at 14 to 15 months of age. Biopsies from VRQ/ARR sheep were negative throughout this period. On the basis of the established or expected incubation period, PrPSc could thus be detected in the tonsils of live susceptible animals at between one-third and a half of the incubation period, more than one-and-a-half years before clinical signs normally appear in both these genotypes.
B E Schreuder; L J van Keulen; M E Vromans; J P Langeveld; M A Smits
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Veterinary record     Volume:  142     ISSN:  0042-4900     ISO Abbreviation:  Vet. Rec.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-08-31     Completed Date:  1998-08-31     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0031164     Medline TA:  Vet Rec     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  564-8     Citation Subset:  IM    
DLO-Institute for Animal Science and Health (ID-DLO), Lelystad, The Netherlands.
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MeSH Terms
Biopsy / veterinary
Disease Susceptibility / veterinary
Genetic Predisposition to Disease
Palatine Tonsil / pathology*
PrPSc Proteins / analysis*,  genetics
Scrapie / diagnosis*
Sheep Diseases / diagnosis*,  genetics
Reg. No./Substance:
0/PrPSc Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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